[1]郭春景,孙长华,王玉芝.快速溶剂萃取-高效液相色谱法测定莎稗磷残留[J].黑龙江农业科学,2011,(07):67-69.
　GUO Chun-jing,SUN Chang-hua,WANG Yu-zhi.Residue Determination of Anilofos in Soil and Rice by Rapid Pressure Organic Solvents Extraction-High Performance Liquid Chromatography[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(07):67-69.
点击复制

快速溶剂萃取-高效液相色谱法测定莎稗磷残留

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2011年07 页码: 67-69 栏目: 出版日期: 2011-07-10
Title:
Residue Determination of Anilofos in Soil and Rice by Rapid Pressure Organic Solvents Extraction-High Performance Liquid Chromatography
文章编号:
1002-2767（2011）07-0067-03
作者:
郭春景1; 孙长华2; 王玉芝2
1.辽宁省农业科学院 开放实验室，辽宁 沈阳 110161；
2.黑龙江省质量监督检测研究院，黑龙江 哈尔滨 150051
Author(s):
GUO Chun-jing1; SUN Chang-hua2; WANG Yu-zhi2
1.Open Testing Center of Liaoning Academy of Agricultural Sciences，Shenyang，Liaoning 110161;
2.The Academy of Quality Inspection and Research in Heilongjiang Province，Harbin， Heilongjiang 150051
关键词:
高效液相色谱; 快速溶剂萃取; 莎稗磷; 残留
Keywords:
high performance liquid chromatography (HPLC); rapid pressure organic solvents extraction; anilofos; residue
分类号:
S481+.8
文献标志码:
A
摘要:
建立了快速溶剂萃取-高效液相色谱法测定土壤、大米中莎稗磷残留量的分析方法。样品经快速溶剂萃取，旋转蒸发，SPE-C18柱净化后，在流动相为甲醇∶水=70∶30，C18柱（250 mm×4.6 mm，5 μm），230 nm，流速1.0 mL·min-1条件下分析测定。结果表明：以甲醇为萃取剂、选择50%的冲池体积选、于100℃萃取10 min，循环1次，10 mL洗脱液进行洗脱的条件下，回收率在90.2%～98.3%，相对标准偏差小于2.89%，检出限为0.1 mg·L-1，线性范围为0.2～10.0 mg·L-1。研究表明该方法操作简单，灵敏度高，干扰小，可满足莎稗磷农药残留检测的要求。
Abstract:
A rapid pressure organic solvents extraction-high performance liquid chromatography method was built to determinate the residual of anilofos in soil and rice.The sample was extracted by organic solvents，rotary evaporation，by C18 solid-phase extraction.The C18 column was used and the mobile phase was a mixture of methanol∶water=70∶30.The detector was VWD and the flow rate was 1.0 mL per minute and the detection wavelength was 230 nm in the HPLC system.The results showed that samples were extracted with methanol，50% of the sample cell，100℃ and 10 min，once circle，10 mL eluent，the recoveries were 90.2%～98.3%.The relative standard deviation was less than 2.89%.The minimum detection was 0.1 mg·L-1.The standard working curve of anilofos had a good linear relation in the concentration range from 0.2～10.0 mg·L-1.This method could meet the requirements of determination because of its simple operation，small interfering and high sensitivity.

参考文献/References:

[1]吕宙明，林永，黄琼辉，等.莎稗磷在稻田系统中的残留动态及最终残留分析[J].福建农林大学学报（自然科学版），2007，36（5）：466-470.

[2]孔祥虹，何强，李春艳，等.气相色谱-质谱法同时检测枸杞子中的克百威、氧环唑、莎稗磷[J].农药，2010，49（3）:188-190.

相似文献/References:

[1]宋伟丰.蛇床子中蛇床子素的提取及检测方法的研究[J].黑龙江农业科学,2013,(02):104.
　SONG Wei-feng.Study on Abstracting and Detecting Method of Osthol in Frutus cnidii[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):104.
[2]孙延芳,梁宗锁,杨开宝,等.高效液相色谱法分析酸枣中的有机酸和维生素C[J].黑龙江农业科学,2011,(08):80.
　SUN Yan-fang,LIANG Zong-suo,YANG Kai-bao,et al.Analysis of Organic Acids and Vc in Sour Jujube byHigh Performance Liquid Chromatography[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(07):80.
[3]王若丁,王 超,孟凡娟,等.工布乌头中三种生物碱HPLC法测定优化[J].黑龙江农业科学,2014,(01):106.
　WANG Ruo-ding,WANG Chao,MENG Fan-juan,et al.The Optimization of HPLC Determination for Three Kinds of Alkaloids in Aconitum kongboense L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):106.
[4]蒋冰清,罗盛东.HPLC法测定滇桂艾纳香中原儿茶酸和原儿茶醛的含量[J].黑龙江农业科学,2015,(03):120.[doi:10.11942/j.issn1002-2767.2015.03.0120]
　JIANG Bing-qing,LUO Sheng-dong.Determination of Protocatechuic Acid and Protocatechuic Aldehyde in Blumeae ripariaeby HLPC[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(07):120.[doi:10.11942/j.issn1002-2767.2015.03.0120]
[5]张鑫喆,赵琳琳,徐方旭,等.HPLC不同流动相测定蛹虫草中虫草素和腺苷的比较[J].黑龙江农业科学,2018,(04):130.[doi:10.11942/j.issn1002-2767.2018.04.0130]
　ZHANG Xin-zhe,ZHAO Lin-lin,XU Fang-xu,et al.Comparison of Cordycepin and Adenosine in Cordyceps Militaris with Different Mobile Phases by HPLC[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(07):130.[doi:10.11942/j.issn1002-2767.2018.04.0130]
[6]陈国峰,尤宏梅,王贵江,等.高效液相色谱法同时测定工业大麻中四种大麻素的方法研究[J].黑龙江农业科学,2021,(11):60.[doi:10.11942/j.issn1002-2767.2021.11.0060]
　CHEN Guo-feng,YOU Hong-mei,WANG Gui-jiang,et al.Simultaneous Determination of Four Cannabinoids in Industrial Hemp by High Performance Liquid Chromatography[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(07):60.[doi:10.11942/j.issn1002-2767.2021.11.0060]
[7]章厉劼,廖欢,栾庆祥,等.HPLC法测定兽用银翘散中绿原酸和连翘苷含量[J].黑龙江农业科学,2022,(10):83.[doi:10.11942/j.issn1002-2767.2022.10.0083]
　ZHANG Li-jie,LIAO Huan,LUAN Qing-xiang,et al.Determination of Chlorogenic Acid and Forsythin in Veterinary Yinqiao Powder by HPLC[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(07):83.[doi:10.11942/j.issn1002-2767.2022.10.0083]

备注/Memo

第一作者简介：郭春景（1982-），女，辽宁省阜新市人，硕士，助理研究员，从事农产品检测及色谱方法研究。E-mail：guocj464@qq.com。

更新日期/Last Update: 2014-10-31