[1]孟庆英.番茄基因组DNA提取探讨[J].黑龙江农业科学,2011,(04):11-12.
　MENG Qing-ying.Study on Extraction of DNA from Tomato[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(04):11-12.
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番茄基因组DNA提取探讨

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2011年04 页码: 11-12 栏目: 出版日期: 2011-04-10
Title:
Study on Extraction of DNA from Tomato
文章编号:
1002-2767（2011）04-0011-02
作者:
孟庆英
黑龙江省农业科学院 佳木斯分院，黑龙江 佳木斯 154007
Author(s):
MENG Qing-ying
Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences，Jiamusi，Heilongjiang 154007
关键词:
番茄; DNA; 提取
Keywords:
tomato; DNA; extraction
分类号:
S641
文献标志码:
A
摘要:
以番茄幼嫩叶片为材料，通过CTAB和SDS 2种方法对番茄基因组DNA提取进行了比较研究。结果表明：2种方法均能提取完整且纯度较高的DNA，所得DNA用于PCR反应可得到清晰的扩增条带。
Abstract:
Taking tender leaves of tomato as materials，the genomic DNA extraction methods of CTAB and SDS from tomato were compared.The results showed that the DNA extracted by CTAB and SDS method both were intact and high purity，and could obtain clear amplified band by PCR.

参考文献/References:

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[2]奥斯伯F，布特伦R，金斯顿R E.精编分子生物学实验指南[M].北京:科学出版社，1998.

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备注/Memo

作者简介：孟庆英（1982-），女，黑龙江省佳木斯市人，硕士，研究实习员，从事土壤肥料与植物基因工程研究。E-mail:MQY269@126.com。

更新日期/Last Update: 2014-10-29