YANG Hong-yi,SUN Li-na,ZHANG Jie.Analysis of Molecular Variation of Cucumber mosaic virus [J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(01):19-21.
黄瓜花叶病毒分子变异分析
- Title:
- Analysis of Molecular Variation of Cucumber mosaic virus
- 文章编号:
- 1002-2767201101-0019-03
- Keywords:
- Cucumber mosaic virus; recombination; phylogenetic analysis
- 分类号:
- S436.421
- 文献标志码:
- A
- 摘要:
- 利用不同生物信息学软件及算法对黄瓜花叶病毒Cucumber mosaic virus，CMV的分子变异进行分析。结果表明：不同CMV分离物外壳蛋白基因的核苷酸序列同源性为76.1%～99.2%。系统进化分析显示不同CMV分离物分为3个大的类群，不同分离物呈现一定的地域相关性，未见明显寄主相关性。重组分析显示澳大利亚分离物243可能为中国分离物PE和韩国分离物Ca-p1的重组体。
- Abstract:
- Molecular variation of Cucumber mosaic virusCMVwas analyzed by bioinformatic software and algorithms.Sequence analysis of coat protein gene showed nucleotide acid identities ranged from 761% to 99.2%.Phylogenetic analysis showed that all isolates of CMV fell into 3 clades.In a certain degree，the clades were related with the geological distributions of CMV，but it was not related with the host origin.Recombine analysis suggested that Australian isolate 243 was likely to be the one of recombine between Chinese isolate PE and Korean isolate Ca-p1.
参考文献/References:
[1]覃瑞，程旺元 . 黄瓜花叶病毒研究进展 [J]. 中南民族大学学报， 2004 ， 232:33 - 37. [2]O’Keefe D C，Berryman D I，Coutts B A，et al.Lack of seed coat contamination with Cucumber mosaic virus?in lupin permits reliable，large-scale detection of seed transmission in seed samples[J].Plant Disease，2007，91:504-508. [3]刘金亮.四种马铃薯Y病毒属病毒的分子变异及HC-Pro结构对抑制RNA沉默的影响[D].泰安：山东农业大学，2007. [4]Li H，Roossinck M J.Genetic bottlenecks reduce population variation in an experimental RNA virus population[J].Journal of Virology，2004，789:10582-10587. [5]Roosinck M J，Zhang L，Hellwald K.Nontranslated region and phylogenetic analyses of Cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups[J].Journal of virology，1999，73:6752-6758.
备注/Memo
基金项目：黑龙江省教育厅科研资助项目(11541033)；中国博士后科学基金资助项目(20080430877)，(200902369)；黑龙江省博士后基金资助项目(LBH-Z08252)