[1]于金海.玉米同义密码子使用偏性分析[J].黑龙江农业科学,2010,(07):1-4.
　YU Jin-hai.Analysis of Synonymous Codon Usage Bias in Maize[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(07):1-4.
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玉米同义密码子使用偏性分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2010年07 页码: 1-4 栏目: 出版日期: 2010-07-10
Title:
Analysis of Synonymous Codon Usage Bias in Maize
文章编号:
1002-2767（201007-0001-04
作者:
于金海
黑龙江省农业科学院 作物育种研究所，黑龙江 哈尔滨 150086
Author(s):
YU Jin-hai
Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences，Harbin，Heilongjiang 150086
关键词:
玉米; 同义密码子使用偏性; 最优密码子; GC含量
Keywords:
maize; synonymous codon usage bias; optimal codons; GC content
分类号:
S513.035.3;Q755
文献标志码:
A
摘要:
利用玉米B73全基因组53 764个基因的表达序列数据，使用多重变量分析软件CodonW对影响密码子用法的因素进行分析。结果表明：在59种同义密码子中有27种为玉米最优密码子。同时还指出，最优密码子的使用频率（FOP与基因的G+C含量（GC，尤其是第3位密码子的G+C百分含量（GC3S、密码子适应指数（CAI和密码子偏爱指数（CBI之间均呈现极显著的正相关（相关系数分别为r＝0.780 7、0.822 3、0.731 4和0.986 3，而与有效等位基因数ENc存在显著负相关（相关系数r=-0.681 5。表明基因GC含量直接影响玉米密码子使用的偏性，同时基因的表达水平越高，对密码子的使用偏向性越强。27个最优密码子的首次确定将对玉米转基因具有重要指导意义。
Abstract:
By using the whole genome sequences of maize inbred B73，twenty-seven optimal codons of maize were identified in 59 synonymous codons and a detailed relative analysis results were obtained by means of a multivariate analysis program-CodonW.The results showed that the frequency of optimal codon (FOP) was present positive correlation with G+C content of genes(GC，r=0.780 7)，G+C content at the third position of synonymous codons(GC3S，r=0.822 3)，codon adaptation index(CAI，r=0.731 4)of species and Codon Bias Index(CBI，r=0.9863).But had negative correlation with effective number of codons(ENc，r= -0.6815).The results indicated that the codon usage of maize genes influenced mostly by GC content of genes with higher expression showed more significant codon usage bias.Twenty-seven codons identified firstly as optimal codons in B73 may provide some more useful information for maize gene-transformation.

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备注/Memo

于金海（1971-，男，黑龙江省密山市人，学士，助理研究员，从事玉米遗传育种研究。E-mail：hnygy@163．com。

更新日期/Last Update: 2014-11-03