ZHAO Hai-hong.Screening of Optimum Medium on Tip Culture of Strawberry Tongzi No.1[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(06):13-15.
童子1号草莓茎尖分化最优培养基筛选研究
- Title:
- Screening of Optimum Medium on Tip Culture of Strawberry Tongzi No.1
- 文章编号:
- 1002-2767(2010)06-0013-03
- Keywords:
- strawberry; stem tip; orthogonal design
- 分类号:
- S668.4
- 文献标志码:
- A
- 摘要:
- 以童子1号草莓茎尖为外植体,以6-苄基腺嘌呤、吲哚丁酸、α-萘乙酸和蔗糖作为主要因素,通过正交设计的方法,对影响草莓茎尖分化的主要因素进行了研究。结果表明:童子1号草莓茎尖分化最适宜的培养基为MS+6-BA 0.5 mg·L-1+IAA 1.0 mg·L-1+蔗糖30 g·L-1+琼脂8 g·L-1,在此条件下草莓茎尖成苗率可达到76.67%。
- Abstract:
- Using stem tip as explants, 6-BA, IAA, NAA and cane sugar as major factors, by orthogonal way combination formula, the main influential factors of stem tip differentiation of strawberry were researched. The results showed that the optimal medium of tip culture was MS+6-BA 0.5 mg·L-1+IAA 1.0 mg·L-1+ cane sugar 30 g·L-1+agar 8 g·L-1. Under these conditions, the seedling ratio could reach 76.67%.
参考文献/References:
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备注/Memo
作者简介: 赵海红(1981-),女,黑龙江省五常市人,硕士,研究实习员,主要从事园艺方面的研究。E-mail: haihong51job@163.com。