YI Cheng,ZHANG Tian-xiao,LIANG Cheng-fu.Studies on Rhizome Culture and Rapid Propagation ofDioscorea Zingi berensis C .H.Wright[J].HEILONGJIANG AGRICULTURAL SCIENCES,2008,(01):11-13.[doi:10.11942/j.issn1002-2767.2008.01.0011]
黄姜根茎组培快繁技术研究
- Title:
- Studies on Rhizome Culture and Rapid Propagation ofDioscorea Zingi berensis C .H.Wright
- 文章编号:
- 1002-2767(2008)01-0011 -03
- 分类号:
- S632 .5
- 文献标志码:
- A
- 摘要:
- 探索了黄姜离体培养快速繁殖的有效途径。结果表明:在附加6 -BA 的MS 培养基上, 外植体可诱导出大量的致密愈伤组织, 而且致密愈伤组织在分化培养基中能分化产生大量圆球茎和幼苗;而在附加2 , 4-D 的MS 培养基中, 外植体被诱导出疏松愈伤组织, 在随后的分化培养基上只能产生数量有限的幼苗。带枝条的圆球茎在MS +NAA0 .5 mg ·L-1 +IAA 0.5 mg · L-1生根培养基上的生根率达到88 .2 %;移栽成活率达85 %。
- Abstract:
- The effective approach of rhizome culture and r apid pr opagation of D ioscorea Zingi berensis C.H .Wri ght w as pr obed into in this paper .The re sults showed tha t:a g reat quantity of compacted ca llus were inducedon the MS medium supplemented with 6 -BA and a larg e amounts of corms and yo ung seedling s w ere diffe rentiatedo n the differentiation medium , but plenty of loo sened callus on the MS medium supplemented with 2 , 4 -D andlimited y oung seedling s o n the differentia tion medium .The roo ting rate o f the corms with branches amo unted to88.2 % on the medium of MS +NAA0.5 mg ·L-1 +IAA0 .5 mg ·L-1 and the t ransplanting survival ratereached 85%.
参考文献/References:
[ 1] 张永忠, 余龙江, 徐辉碧.黄姜的研究进展[ J] .中药材, 2003 , 26(5):376-378 .
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备注/Memo
基金项目:湖南教育厅科技计划项目(04C027);湖南省科技厅科技计划项目(01JZY2095)