MIN Fan-xiang,HU Lin-shuang,WANG Xiao-dan,et al.Comparison Study of the ELISA Kit Made in China and USA for Detecting Potato Ring Rot[J].HEILONGJIANG AGRICULTURAL SCIENCES,2007,(05):57-59.[doi:10.11942/j.issn1002-2767.2007.05.0057]
自制和进口试剂盒检测马铃薯环腐病的比较研究
- Title:
- Comparison Study of the ELISA Kit Made in China and USA for Detecting Potato Ring Rot
- 文章编号:
- 1002 - 2767(2007)05 - 0057 - 03
- 关键词:
- 马铃薯环腐病; NCM - ELISA ; DAS - ELISA
- Keywords:
- Patato Ring Rot ; NCM - ELISA technique ; DAS - ELISA technique
- 分类号:
- S435. 32
- 文献标志码:
- A
- 摘要:
- 采用自制NCM - ELISA 试剂盒和进口DAS - ELISA 试剂盒进行马铃薯环腐病检测试验。比较发现:强化后的自制NCM - ELISA 试剂盒不仅同进口DAS - ELISA 试剂盒一样灵敏, 而且价格低廉, 且简便、快捷。自制NCM - ELISA 试剂盒可以将点样后的硝酸纤维素膜存储数周, 然后再继续实验, 具有便于携带利于推广的优点。而且自制NCM - ELISA 试剂盒在检测前, 进行富集培养, 与进口DAS - ELISA 试剂盒相比将灵敏度提高100 万倍, 并且避免假阳性反应发生。因此NCM - ELISA 试剂盒完全可以取代进口DAS - ELISA 试剂盒。
- Abstract:
- Applying NCM ELISA Kit and DAS ELISA Kit to Detect Potato Ring Rot w as com par ed. The results showed that the intensifying NCM ELISA wa s as sensitive a s the DAS ELISA , but also cheap , inco nv enient and r apid. I t had ano ther im po rtant adva ntag e , that w as , the nitrocellulo se membr ane with the sample s could be sto red for several w eeks befo re continuing with the test. The membr ane could also be sent to ano ther labo rato ry that co uld perfo rm the assay , and it w as easier and quicker befo re pe rfo rming the NCM ELIS A, an enrichment procedure must be car ried o ut by incubating the tube r ex trac ts in a semi-selective bro th (modified SM SA). This me tho d increased the sensitivity by almo st 1 million hig her tha n DAS ELISA Kit , and avo ided artificial positive reactio n. Therefo re , NCM ELISA Kit could re place DAS ELISA Kit.
参考文献/References:
[ 1] 孙秀梅. 马铃薯环腐病的发生及其防治[ J] . 农业科技通讯, 2001(1):26-26.
[ 2] Slack S A , Drennan J L , West ra A A G. C om pari son Of PCR , ELISA an d DNA hy bridizati on f or the det ecti on of Cl avibact er michiganensi s subsp. sepedonicu s in field g row n pot at oes[ J] . Plant Di sease , 1996 , 80 :519-524.
[ 3] 高虹. 马铃薯环腐病的发生及防治[ J] . 现代化农业, 2004(1): 10-11.
[ 4] 何云霞, 张儒喜, 白艳菊, 等. 马铃薯环腐病菌鉴定检测技术研究进展[ J] . 中国马铃薯, 2004 , 18(3):159-162.
[ 5] 周艳玲, 刘学敏, 孟玉芹. 马铃薯Y 病毒的检测技术[ J] . 中国马铃薯, 2000 , 14(2):89-93.
[ 6] Salazar L F. S erol ogi cal meth ods f or virus detect ion. In potat o viruses an d their cont rol[ C] . Lima(PE):Internati onal Potat o Center , 1996 :113-132.
[ 7] 王乐义, 马洪. 自制和进口试剂盒检测鸡传染性支气管炎抗体的比较研究[ J] . 安徽农业科学, 2005 , 33(9):1665-1713.
[ 8] 李汝刚, 郭军, 鞠振林, 等. 马铃薯X 、Y 病毒的单克隆抗体及其在马铃薯病毒检测上的应用[ J] . 马铃薯杂志, 1992 , 6(4): 233-236.
备注/Memo
收稿日期:2007 - 06 - 20
基金项目:黑龙江省自然科学基金项目(2004C 0307)
第一作者简介:闵凡祥(1980 —), 男, 黑龙江嫩江县人, 学士, 研实, 主要从事马铃薯真细菌病害研究。E - mail:minfanxiang. m a @yah oo. com. cn 。