[1]王黎明.外源D N A 导入高粱的叶部病害变异及其分子验证[J].黑龙江农业科学,2007,(03):1-3.[doi:10.11942/j.issn1002-2767.2007.03.0001]
　WANG Li-ming.Leaf Diseases Resistance Variation and Its RAPD Check of Sorghum Transferred Progenies by Exogenous DNA Introduction[J].HEILONGJIANG AGRICULTURAL SCIENCES,2007,(03):1-3.[doi:10.11942/j.issn1002-2767.2007.03.0001]
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外源D N A 导入高粱的叶部病害变异及其分子验证

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2007年03 页码: 1-3 栏目: 出版日期: 2007-05-10
Title:
Leaf Diseases Resistance Variation and Its RAPD Check of Sorghum Transferred Progenies by Exogenous DNA Introduction
文章编号:
1002-2767(2007)03-0001-03
作者:
王黎明
黑龙江省农科院作物育种所, 哈尔滨1 5 0 0 8 6
Author(s):
WANG Li-ming
Crop Breeding Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086
关键词:
高粱;  外源D N A 导入;  叶部病害;  分子脸证
Keywords:
sorghum; exogenous DNA introduction;  leaf diseasesf RAPD check
分类号:
S514.034
DOI:
10.11942/j.issn1002-2767.2007.03.0001
文献标志码:
A
摘要:
通过花粉管通道, 将杭叶部病害能力强的热带高粱D N A 导入后, 有27 个导入后代的扰病性有所提高, 占导入后代的9 . 1% 。其中有5 个导入后代的杭病性提高了2 级, 3 个导入后代的杭病性提高了3 级, 显著地提高了受体的杭病性。表明供体D N A 片段已通过花粉管通道进入受体, 并对受体基因的表达产生影响。此项技术实现了地理远缘的品种间的遗传物质转移, 丰富了遗传类型。
Abstract:
Exogenous DNA with leaf diseases resistance of tropical sorghum was introduced through pollen tube pathway. 27 transferred progenies’ diseases resistance were mcreased, accounting for 9. 1% ,in which 5 transferred progenies’ diseases resistance increased 2 grade and 3 transferred proge- nies’ diseases resistancelncreased 3 grade. The disease resistance of receptor was increased significant- ly. This indicated that,donor DNA fragment had inserted into receptor through pollen tube, and af- fected the expression of genes in receptors. This technique realized the transference of inheritable mat- ter between varieries of distant geography, and made the inheritable types abundant.

参考文献/References:

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备注/Memo

收稿日期：2006-12-05
基金项目：国家“863”项目(2001AA241232)
第一作者简介：王黎明（1968-），女，哈尔滨市人，硕士，副研究员，从事高粱育种研究。E- mailIdawnw@126. com

更新日期/Last Update: 2016-12-18