[1]李国良.热导系玉米种子贮存蛋白的生物指纹图谱分析[J].黑龙江农业科学,2005,(06):25-27.[doi:10.11942/j.issn1002-2767.2005.06.0025]
　LI Guo-liang.Finger Print Analysis on Seed Protein of Maize Inbred Line[J].HEILONGJIANG AGRICULTURAL SCIENCES,2005,(06):25-27.[doi:10.11942/j.issn1002-2767.2005.06.0025]
点击复制

热导系玉米种子贮存蛋白的生物指纹图谱分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2005年06 页码: 25-27 栏目: 出版日期: 2005-11-10
Title:
Finger Print Analysis on Seed Protein of Maize Inbred Line
文章编号:
1002-2767(2005)06-0025-03
作者:
李国良
Author(s):
LI Guo-liang
Maize Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086
关键词:
玉米; 聚丙稀酰胺凝胶电泳; 指纹图谱
Keywords:
maize;  SDS-PAGE;  finger print
分类号:
S513
DOI:
10.11942/j.issn1002-2767.2005.06.0025
文献标志码:
A
摘要:
利用聚丙稀酰胺凝胶电泳(SDS-PAGE)对热导系玉米种子的可溶性蛋白进行分析，结果表 明，清蛋白、球蛋白、谷蛋白都显示出丰富的带谱。自交系间谱带数目、带的强弱与分布等特点均可 见较明显的差异，可将各供试自交系互相区分出来；醇溶蛋白谱带均较弱，自交系间差异小，难以把 各供试自交系互相区分出来。尽管种子的可溶蛋白质 PAGE 图谱都可作为玉米自交系鉴定的蛋 白质指纹图谱，相比之下，利用清蛋白 PAGE 图谱较为理想。
Abstract:
Finger Print Analysis on Seed Protein of Maize Inbred Line1002-2767(2005)06-0025-03Abstract: Electrophoretic analysis was made on the seed soluble protein of 9 maize inbred lines bypolyacryamide gel electrophoresis (PAGE) techniques . The results showed that the zeins wereboth simple and differences among varieties were small. It was ,therefore, difficult to distinguishall the varieties studied with the zein patterns, Though, the PAGE of the seed soluble proteins allcan be regarded as protein fingerprint for identification of 9 maize varieties, the PAGE patterns ofthe albumins were ,however, abundarit and the differences among varieties were obvious in bandsnumber distribution and concentration . On the basis of these characteristics ,all the varietiesstudied could be discriminated from each other .It was suggested that PAGE of seed albumin wasuseful in variety identification in maize.

参考文献/References:

[1]粱明山，侯留记．聚丙烯酰胺凝胶电泳鉴定烟草品种[J]．种 子,2001,(1)：9—11．[2]颜廷进，李群，公茂洪．玉米种子蛋白质电泳技术的改进[J].种 子科技．1999，(2):28-29．

[3]颜启传，王未英，周亚娣．杂交玉米丹玉13及其亲本自交系品 种的同工酶电诛鉴定[J].浙江农业大学学报．1993，19(3)： 313-315．
[4]宋同明，郑大浩，刘岩．利用玉米种子清蛋白和球蛋白乳酸聚丙 烯酰胺电泳鉴定品种[J]．植物学报，1996,38 (8)：599-604
[5]张春庆，金锡奎，郑成超．NAU - PAGE技术与玉米种子纯 度测定[J].中国农业科学，1995，28(6) ,20-24．
[6] Mcdonald MB t Elliotj, Sweeney PM. DNA extraction from dry seeds for RAPD analyses in varietal identification studies [J]. Seed SCl and Techn01,1994 ,22(1):171-176.
[7] Snuth J S C, Smith O&Restriction fragment length polymor- phism can differentiate among U.S.maize hybrids[J]. Crop Scl,1991,31:893-899。
[8]胡表昂，王洪新．蛋白质多样性和品种鉴定[J].植物学报t 1991,33(7):556-564．
[9]王景升，王玺，刘国奇，等，电泳谱带法在作物品种纯度鉴定上 的应用研究[J]．沈阳农业大学学报，1996,27(3) ,221-225．
[10]王玺，张野田，玉米杂交种纯度检测方法及其述评[J].种子． 1997,(3)131-34．
[11] C．札Wilson. Seed Protein Fraction of Maize, Sorghum and Related Cereals[J]. Seed Proteins,1983,3(5) ,271-307.
[12]朱应泽，刘容山，李方安，玉米种子蛋白质电泳鉴定品种的研 究[J]．四川农业大学学报．1995，13(2)：150-153．

相似文献/References:

[1]王静.西昌地区玉米自交系苗期耐低磷特性鉴定[J].黑龙江农业科学,2013,(02):5.
　WANG Jing.Identification of Resistance to Low-phosphorus Characteristics of Maize Inbred Lines at Seedling Stage in Xichang Area[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(06):5.
[2]石绍河,符强,关晶.控释肥对玉米产量及效益的影响研究[J].黑龙江农业科学,2013,(02):44.
　SHI Shao-he,FU Qiang,GUAN Jing.The Effect of Controlled-Release Fertilizer on Maize Yield and Benefit[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(06):44.
[3]石凤梅,马立功.黑龙江省玉米大斑病菌小种生理分化的研究[J].黑龙江农业科学,2013,(02):63.
　SHI Feng-mei,MA Li-gong.Study on Physiological Race Differentiation of Exserohilum turcicum in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(06):63.
[4]马延华,孙德全,李绥艳,等.玉米自交系灌浆期抗倒性评价及抗倒性状的相关分析[J].黑龙江农业科学,2014,(07):5.
　MA Yan-hua,SUN De-quan,LI Sui-yan,et al.Evaluation of Lodging Resistance and Correlation Analysis of Lodging Resistance Characters for Maizenbred Lines During Grain filling Period[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(06):5.
[5]杨耿斌,刘兴焱,王立春,等.黑龙江早熟地区深松对玉米产量及土壤状态的影响[J].黑龙江农业科学,2014,(07):23.
　YANG Geng-bin,LIU Xing-yan,WANG Li-chun,et al.Effect of Subsoiling on Maize Yield and Soil Characteristics in Earlymaturing District of Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(06):23.
[6]王宇先.寒地半干旱地区膜上精播技术对玉米生长发育的影响[J].黑龙江农业科学,2014,(07):29.
　WANG Yu-xian.Effect of Planting in Mulching Film on the Growth and Development of Maize Plant in Cold and Semi Arid Area[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(06):29.
[7]刘志华,盖兆雪,李晓梅,等.秸秆还田对玉米产量形成及土壤肥力的影响[J].黑龙江农业科学,2014,(07):42.
　LIU Zhi-hua,GAI Zhao-xue,LI Xiao-mei,et al.Effect of Straw Return on Maize Yield Components and Soil Fertility[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(06):42.
[8]林红,何晶丽.玉米在畜牧养殖业中的有效利用[J].黑龙江农业科学,2014,(07):77.
　LIN Hong,HE Jing-li.Effective Utilization of Maize in Animal Husbandry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(06):77.
[9]林 红,孙德全,李绥艳,等.玉米单倍体诱导系对不同类群F1的诱导率评价[J].黑龙江农业科学,2014,(06):12.
[10]张崎峰,蔡鑫鑫,李金良,等.黑龙江省高纬寒地大垄双行栽培模式下玉米品种筛选试验[J].黑龙江农业科学,2014,(06):22.
　ZHANG Qifeng,CAI Xinxin,LI Jinliang,et al.Screening Test of Maize Varieties under the Mode of Double Row Sowing with Big Ridge in High Latitude and Cold Region of Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(06):22.

备注/Memo

收稿日期：2005-07-14

作者简介：李国良（1973-），男，黑龙江省肇东市人，助理研究员，从事玉米育种研究。

更新日期/Last Update: 2016-12-09