[1]南相日,刘文萍,韩玉琴,等.PLO 介导PLRV -CP 基因转化马铃薯获转基因抗性植株[J].黑龙江农业科学,2004,(03):1-3.[doi:10.11942/j.issn1002-2767.2004.03.0001]
　NAN Xiang-ri,LIUWen-ping,HAN Yu-qin,et al.PLRV-CP Gene Transferring Potato Gain Disease Resistant Transplant by Poly -L-Ornithine[J].HEILONGJIANG AGRICULTURAL SCIENCES,2004,(03):1-3.[doi:10.11942/j.issn1002-2767.2004.03.0001]
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PLO 介导PLRV -CP 基因转化马铃薯获转基因抗性植株

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2004年03 页码: 1-3 栏目: 出版日期: 2004-05-10
Title:
PLRV-CP Gene Transferring Potato Gain Disease Resistant Transplant by Poly -L-Ornithine
文章编号:
1002 -2767(2004)03 -0001 -03
作者:
南相日1 ;  刘文萍1 ;  韩玉琴1 ;  黄金亮2 ;  张春安2
1.黑龙江省农科院生物技术研究中心,哈尔滨150086 ;
2.敦化市马铃薯繁育开发中心, 敦化135000
Author(s):
NAN Xiang-ri1 ;  LIUWen-ping1 ;  HAN Yu-qin1 ;  HUANG Jin-l iang2 ;  ZHANG Chun-an2
1 .Heilongjiang Academy of Agricultural Sciences Harbin 150086 ;
2 .Dunhua Po tato Breeding andDevelopment Center Dunhua 135000
关键词:
马铃薯; 原生质体; PLRV -CP 基因
Keywords:
potato ; protoplast ; PLRV -CP gene
分类号:
S532 .035.3
DOI:
10.11942/j.issn1002-2767.2004.03.0001
文献标志码:
A
摘要:
利用PLO(Poly -L -Ornithine)将外源PLRV -CP 基因导入到马铃薯加工型品种大西洋原生质体中, 获得了转化后代, 经过PCR 和RT -PCR 分析均显阳性, 蚜虫接种PLRV , 结果比对照明显抗PLRV.。
Abstract:
The ex ternal PLRV -CP gene w as t ransferred into processing Potato pro toplasts and gottransferred progeny by PLO .The PCR and RT -PCR analysis w as positive .After inoculating PLRVw ith aphid , the transplant was much more resistant to PLRV than cont rol .

参考文献/References:

[ 1]张成良, 张作芳.植物病毒的分类和命名[ M] .北京:中国农业出版社, 1996.124-127 .

[ 2]J .萨姆布鲁克.E .F.费里奇.T .曼尼阿蒂斯.分子克隆实验指南[ M] .北京:科学出版社, 1992.19-22 .
[ 3]卫志明, 许智宏.大豆原生质体培养和植株再生[ J] .植物学报, 1990 , 32(8):582-588 .
[ 4]南相日, 卫志明.利用聚鸟氨酸提高大豆原生质体外源基因的转化效率[ J] .实验生物学报,1999 , 32(4):409-413 .
[ 5]傅荣昭, 孙勇如, 贾士荣.植物遗传转化技术手册[ M] .北京:中国科学技术出版社,1994 .168-170 .
[ 6]S tewart C N Jr .Via LE A rapid CTAB DNA isolat ion techniqueuseful for RAPD fingerprint ing and other PC R applications[ J] .Biot echniques , 1993, 14 :748-751.

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备注/Memo

收稿日期:2004 -02 -12　

基金项目:省科委攻关资助项目(GA01B101 -08)　
第一作者简介:南相日(1966 -), 男, 吉林省辉南县人, 1991 年毕业于东北农学院生物工程系植物生理生化专业, 获硕士学位。毕业后在黑龙江省农业科学院生物技术研究中心一直从事生物技术工作。

更新日期/Last Update: 2016-12-07