[1]雷 蕾,康庆华,张晓波,等.反相高效液相色谱法分离和测定亚麻植株中植物激素[J].黑龙江农业科学,2001,(06):21-22.[doi:10.11942/j.issn1002-2767.2001.06.0021]
LEI Lei,KANG Qing-hua,ZHANG Xiao-bo,et al.Separation and Determination of Plants Hormones in FLax Plant With Reversed -phase High Efficient Liquid Chromatography[J].HEILONGJIANG AGRICULTURAL SCIENCES,2001,(06):21-22.[doi:10.11942/j.issn1002-2767.2001.06.0021]
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LEI Lei,KANG Qing-hua,ZHANG Xiao-bo,et al.Separation and Determination of Plants Hormones in FLax Plant With Reversed -phase High Efficient Liquid Chromatography[J].HEILONGJIANG AGRICULTURAL SCIENCES,2001,(06):21-22.[doi:10.11942/j.issn1002-2767.2001.06.0021]
反相高效液相色谱法分离和测定亚麻植株中植物激素
《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S]
卷:
期数:
2001年06
页码:
21-22
栏目:
出版日期:
2001-11-10
- Title:
- Separation and Determination of Plants Hormones in FLax Plant With Reversed -phase High Efficient Liquid Chromatography
- 文章编号:
- 1002 -2767(2001)06 -0021 -02
- Keywords:
- flaxp lant s ; indole -3 -acetic acid(IAA); gibberellic acid(GA3); liquid chromatography ; separation and determinatied
- 分类号:
- S563 .2
- 文献标志码:
- A
- 摘要:
- 探讨了用反相高效液相色谱法分离和测定经不同除草剂处理的亚麻植株中两种生长激素吲哚已酸(IAA)与赤霉素(GA3)的方法。以甲醇、乙腈和水为流动相, 5 分钟可将两种激素快速分离和定量测定, 线性关系良好, 回收率较高。
- Abstract:
- In this paper , reversed -phase high ef ficient liquid chromatography w as used for the separatio n and determinat ion of plant hormones indole -3 -acetic acid(IAA)and gibberellic acid(GA3)in flax plantstreated w ith dif ferent herbicides .Methanol , acetonit ril and water system w as chosen as the mobile phase .Within 5 minutes two plant hormones can be separated and determinated .The calirat ion curves for the two compounds had a lineal relat ion .The rete of recycle was very hig h .
参考文献/References:
[ 1] 吴桐.生物化学[ M] .北京:人民教育出版社, 1987.
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备注/Memo
收稿日期:2001 -05 -28
作者简介:雷蕾(1968 -), 女, 哈尔滨市人, 工程师, 现从事化学分析工作。
更新日期/Last Update:
2016-12-06