Wang Guangjin.Prel iminary Study on Cross Between H. vulgare ( 2x) and H. bulbosum ( 4x)[J].HEILONGJIANG AGRICULTURAL SCIENCES,2000,(03):1-2.[doi:10.11942/j.issn1002-2767.2000.03.0001]
二倍体栽培大麦与四倍体野生大麦杂交的研究初报
- Title:
- Prel iminary Study on Cross Between H. vulgare ( 2x) and H. bulbosum ( 4x)
- 文章编号:
- 1002- 2767( 2000) 03- 0001- 02
- Keywords:
- hordeum vulgare; hordeum bulbosum ; cross
- 分类号:
- S512. 303. 51
- 文献标志码:
- A
- 摘要:
- 二倍体栽培大麦与四倍体野生大麦杂交产生的杂种除三倍体外还有非整倍体,后代分离出栽培大麦类型、野生大麦类型和中间型。酯酶同工酶分析结果表明,除具有双亲谱带外,还有新的谱带产生,这可能与非整倍体的存在有关。辐照杂种愈伤组织的适宜剂量为5Gy左右。
- Abstract:
- Among the hybrids derived from crosses betw een H. vulgare ( 2x ) and H. bulbosum ( 4x ) , there w ere not only t riploids but also aneuploids. The elect ropho resis analy sis of esterase ( Est ) isozym w as perfo rmed for hy brids as well as their parents. The result show ed that there w ere different bands among hybrid plants. Besides some bands w hich w ere present in both parent s, a new band was visible between Est1 and Est2 in some plants. This may be due to the existence of aneuploids. The optimum doses for irradiating hybrid calli was about 5 Gy.
参考文献/References:
[1 ]Noda and hash a, Ch romosome eliminati on in t riploid betw een H. v ulgare ( 2x ) and H. bulbosum ( 4x ) [ J] . Cereal Res earch Communi cation, 1981, ( 9): 85～ 91.
[2 ]Pickering R. A. Th e assessment of variation in two population of H. bulbosum for improving s uccess rate in a doubled h aploid barly programme [ J]. Euph ytica. 1983, ( 32): 903～ 910.
[3 ]Pickering R. A. partial Cont rol of ch romosome elimination by temperature in immature em bryos of H. vulgare X H. bulbosum [ J ]. Euphyti ca, 1985, ( 43): 869～ 874.
[ 4 ]Pick ering R. A. Th e p roducti on of f ertile triploid hybrids betw een H. vulgare ( 2n= 2x= 14) and H. bulbosum ( 2n= 4x= 28) [ J] . Barley Geneti cs New slet ter, 1988a, ( 18): 25～ 29.
[5 ]Yu ji e and J. W. Snape Th e cytolog y of hybrids betw een H. vulgare and H. bulbosum revi si ted [ J ]. Genome, 1988, ( 30): 486～ 494.
[ 6 ]Sh aw , C. R. and R. Prasad Starch gel el ect rophoresis of enzymes a compilation of recipes [ J ] , Bioch. Genet , 1970, ( 4): 297～ 320.
备注/Memo
收稿日期: 1999- 12- 14
作者简介: 王广金( 1962- ) ,男,副研究员,在读博士,从事小麦育种研究。