LIU Qijun,ZHANG Zhiyang,XU Kuan,et al.Antibacterial Efficacy of Hengjingsuan and Its Impact on Intestinal Flora in Mice[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(03):30-38.[doi:10.11942/j.issn1002-2767.2024.03.0030]
恒净酸的抑菌效果及对小鼠肠道菌群的影响
- Title:
- Antibacterial Efficacy of Hengjingsuan and Its Impact on Intestinal Flora in Mice
- 文章编号:
- 6
- Keywords:
- Hengjingsuan; bactericidal efficacy; intestinal flora
- 文献标志码:
- A
- 摘要:
- 为探讨以枸橼酸、苹果酸、氨基磺酸、甲酸等为主要成分的恒净酸杀菌效果和对小鼠肠道微生物菌群的影响,采用悬液定量杀菌试验等方法观察恒净酸对大肠杆菌、白色念珠菌、金黄色葡萄球菌、铜绿假单胞菌和非洲猪瘟病毒的杀菌效果;采用14 d最大给药量法观察恒净酸的急性毒性反应;通过观察不同稀释度恒净酸对HEK293T细胞形态的影响确定其对细胞的安全浓度;采用高通量测序技术分析恒净酸对小鼠肠道细菌群落结构多样性的影响。结果表明,0.1 g·L-1恒净酸溶液2 min 内可有效杀灭大肠杆菌、白色念珠菌、金黄色葡萄球菌、铜绿假单胞菌;1.56 g·L-1恒净酸溶液5 min后可有效灭活非洲猪瘟病毒。恒净酸为实际无毒级物质,对细胞的安全浓度为≤1.56 g·L-1。与对照组相比,恒净酸处理显著提高了拟杆菌门的相对丰度,提高了拟杆菌门/厚壁菌门的比例。综上,恒净酸是一种可有效杀灭病原微生物的新型消毒剂,能改善小鼠的肠道菌群组成,且安全无毒。
- Abstract:
- To observe the bactericidal efficacy of Hengjingsuan mainly composed of citric acid, malic acid, sulfamic acid and formic acid and its impact on the intestinal microbiota in mice. Suspension quantitative bactericidal test was used to evaluated the bactericidal efficacy of Hengjingsuan on Escherichia coli8099, Candida albicans ATCC 10231, Staphylococcus aureus CMCC(B) 26003, Pseudomonas aeruginosa ATCC 15442 and African Swine Fever Virus (ASFV). The acute toxicity test of Hengjingsuan in mice were was carried out by 14 days maximum dose method and the safe concentration of Hengjingsuan was determined by observing the effect of disinfectant on cell morphology of HEK293T cells and calculating the cell activity. The effect on community structure diversity of intestinal bacteria was analyzed by using high-throughput sequencing technology.The results showed that Hengjingsuan solution at the concentration of 0.1 g·L-1 could kill E. coli 8099, C. Albicans ATCC 10231, S. aureus CMCC(B) 26003, P. aeruginosa ATCC 15442 effectively within 2 minutes, and it could kill ASFV effectively at 1.56 g·L-1 within 5 minutes. Hengjingsuan was an actual non-toxic substance, and its safe concentration for the cell was ≤ 1.56 g·L-1. Compared with normal group, the abundance of bacteroidetes significantly increased in mice treated with Hengjingsuan, meanwhile the abundance ratio of bacteroidetes to Firmicutes also increased. Therefore, Hengjingsuan was a new type of disinfectant, which was safe, non-irritating and had great bactericidal efficacy on target pathogenic microorgan.
参考文献/References:
[1]林冬梅,高计会,唐茂妍.饮水酸化剂在畜禽生产中的应用[J].中国家禽,2019,41(10):71-73.[2]李其松,吴春阳,吴玉龙,等.猪场饮水生物安全浅析[J].猪业科学,2020,37(9):84-86.[3]杨有福,李想,毕小娟.非洲猪瘟新阶段下的猪场水环境防控[J].今日养猪业,2020(3):66-68.[4]游武进.大型养殖场水线管理[J].今日养猪业,2023(1):55-56.[5]黄明.畜禽养殖场的饮水消毒[J].水禽世界,2015(1):46-47.[6]梁娜,梁思嘉,冯芳.不同消毒剂对肉鸡水线消毒效果对比实验[J].今日畜牧兽医,2017(7):10.[7]赵学峰,王然,刘晓辉,等.酸化剂在猪生产中的应用[J].中国动物保健,2021,23(5):70-71.[8]丑有财,蒋国政.酸化剂在动物饮水中的应用价值[J].黑龙江畜牧兽医,2014(12):53-54.[9]柴倩,张瑞芳,冯泽伟,等.酸化剂对猪、鸡肠道健康影响的研究进展[J].饲料研究,2023,46(13):150-154.)[10](王鑫源,张莹,董晓雪,等.酸化剂在猪生产中的应用研究进展[J].今日畜牧兽医,2023,39(5):76-78.[11]任方奎,张桂芝,张燕平,等.液体酸化剂的评估及其对肉鸡生长性能的影响[J].饲料研究,2021,44(1):49-52.[12]于睿,胡颖,黎明,等.饮水型酸化剂对蛋鸡生产性能·蛋品质及输卵管健康的影响[J].安徽农业科学,2021,49(7):92-96.[13]国家质量监督检验检疫总局.一次性使用卫生用品卫生标准:GB 15979─2002[S].北京:中国标准出版社,2002.[14]中华人民共和国国家卫生和计划生育委员会.食品安全国家标准急性经口毒性试验:GB 15193.3─2014[S].北京:中国标准出版社,2015.[15]曾献春,朱雅琴,王明明,等.干酪乳杆菌对便秘小鼠肠道菌群的影响[J].食品与发酵工业,2022,48(6):64-70.[16]李建华,赵良友,张娜,等.白桦茸不同提取物对小鼠急性毒性实验研究[J].长春中医药大学学报,2021,37(1):71-75.[17]张楠,李武,彭慧娟,等.吸水链霉菌多糖的纯化鉴定及其性质[J].核农学报,2017,31(12):2367-2376.[18]张斌,瞿涛.规模化养猪场保育猪管理技术[J].畜禽业,2023,34(8):57-59.[19]何纪垲,邹新华,张增玉,等.饮水中添加酸化剂对产蛋后期蛋鸡产蛋性能、血清生化指标及抗氧化能力的影响[J].饲料研究,2023,46(9):42-46.[20]祁梦南,潘力,孙元,等.抗低温消毒剂“抗冻消-30”对非洲猪瘟病毒的杀灭效果[J].中国预防兽医学报,2021,43(9):972,977,1007.[21]张凯军,姜鹏飞,王军,等.不同温度对中华绒螯蟹生长及肠道微生物菌群的影响[J].上海海洋大学学报,2022,31(2):384-393.[22]姬妍茹,张正海,杨庆丽,等.黑菊芋多糖的润肠通便和调节肠道菌群作用[J].现代食品科技,2022,38(7):33-39.[23]金旭,吴琼,李祯,等.益生菌与酸化剂联用对肉鸡生长性能、抗氧化能力和肠道健康的影响[J/OL].动物营养学报,2023,35(12):1-12(2023-10-13)[2023-10-30].https://link.cnki.net/urlid/11.5461.S.20231013.1357.020.
备注/Memo
收稿日期:2023-10-17