WU Yue,LU Yuantian,MAO Mingjuan,et al.Optimization of Cultivation Conditions for Pleurotus sapidus[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(07):67-74.[doi:10.11942/j.issn1002-2767.2024.07.0067]
紫孢侧耳培养条件的优化研究
- Title:
- Optimization of Cultivation Conditions for Pleurotus sapidus
- 文章编号:
- 12
- Keywords:
- Pleurotus sapidus; growth characteristics; culture medium
- 文献标志码:
- A
- 摘要:
- 紫孢侧耳是食药兼用真菌，营养丰富且具有多种药用功效，为了进一步开发这一自然资源，通过单因素试验和正交试验对实验室保存的一株紫孢侧耳菌种进行生长特性研究，确定培养基的最佳配方。结果表明，在6种供试碳源中，紫孢侧耳菌丝以利用多糖即淀粉生长最好，淀粉最佳添加量为20 g·L-1；在6种供试氮源中，菌丝在有机氮源中的长势更好，其中以添加酵母浸粉的长势最佳，酵母浸粉最佳添加量为8 g·L-1；MgSO4添加量对菌丝的生长影响较小，则MgSO4最适添加量为0.2 g·L-1；KH2PO4添加量对菌丝的生长影响不显著，则KH2PO4添加量为0.5 g·L-1；菌丝在pH5～9范围内均能正常生长，最适pH为7。
- Abstract:
- Pleurotus sapidus (P. sapidus), a valuable edible and medicinal fungus, possesses abundant nutritional and medicinal properties. In order to further explore P.sapidus, this study aimed to investigate the growth characteristics of a laboratory-preserved strain of P. sapidus through single-factor experiments and orthogonal experiments to determine the optimal culture medium formula. The results revealed that among the six tested carbon sources, P. sapidus mycelium exhibited the best growth when utilizing polysaccharides, particularly starch, with an optimal addition amount of 20 g·L-1. Among the six tested nitrogen sources, organic nitrogen sources were more favorable for mycelial growth compared to inorganic nitrogen sources, with yeast extract powder being the most optimal nitrogen source and an addition amount of 8 g·L-1. The addition amount of MgSO4 had a minor influence on mycelial growth, therefore it was determined to be 0.2 g·L-1. The effect of KH2PO4 on mycelial growth was not significant, thus an addition amount of 0.5 g·L-1 was determined. P. sapidus mycelium was able to grow normally within the pH ranged from 5 to 9, with an optimal pH value of 7.
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备注/Memo
收稿日期：2023-11-20