[1]于晓丽,王培松,张 伟,等.烟台地区小麦纹枯病病原菌群体组成与致病力分化[J].黑龙江农业科学,2017,(04):43-47.[doi:10.11942/j.issn10022767.2017.04.0043]
　YU Xiaoli,WANG Peisong,ZHANG Wei,et al.Composition and Virulence Variation of the Pathogen of Wheat Sharp Eyespot from Yantai[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(04):43-47.[doi:10.11942/j.issn10022767.2017.04.0043]
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烟台地区小麦纹枯病病原菌群体组成与致病力分化

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年04期 页码: 43-47 栏目: 出版日期: 2017-04-10
Title:
Composition and Virulence Variation of the Pathogen of Wheat Sharp Eyespot from Yantai
文章编号:
10022767（2017）04004305
作者:
于晓丽; 王培松; 张 伟; 陈 敏; 王英姿
山东省烟台市农业科学研究院，山东 烟台 265500
Author(s):
YU Xiaoli; WANG Peisong; ZHANG Wei; CHEN Min; WANG Yingzi
Yantai Academy of Agricultural Science,Yantai，Shandong 265500
关键词:
小麦纹枯病; 生长速率; 致病力; 融合群
Keywords:
wheat sharp eyespot;  growth rate;  pathogenicity;  anastomosis groups
分类号:
S435.121.4+9
DOI:
10.11942/j.issn10022767.2017.04.0043
文献标志码:
A
摘要:
为了促进小麦纹枯病的防治，从烟台各县市区共采集分离小麦纹枯病菌菌株40个，通过细胞核染色、菌丝融合反应、rDNAITS序列分析测定得到的纹枯病菌的群体组成；通过室内试验测定菌株生长速率及致病力，比较不同地区小麦纹枯病菌的生长速率和致病力差异，并对其相关性进行分析。结果表明：烟台纹枯病菌株属于AGD、AG4和AG5三个融合群，分别占总菌株数的92.5%、5.0%和2.5%；小麦纹枯病菌的生长速率在5.33~13.00 mm?d，分为快速、中速和慢速三种生长类型，分别占总株数的55.0%、40.0%和5.0%；随机选取的AGD融合群20个菌株通过室内试验测定其致病力，病情指数在16.67%~68.25%，分为强毒力、中等毒力和弱毒力菌株三个等级；进一步的线性分析发现烟台地区小麦纹枯病菌生长速率与致病力之间成中等程度正相关性。
Abstract:
In order to promote the prevention and control of wheat sheath blight,a total of 40 Rhizoctonia isolates from different counties of Yantai was collected and isolated,the anastomosis groups by nuclear staining,hyphal fusion reaction and rDNAITS sequence analysis were determined,the growth rate and pathogenicity of different strains through measuring were compared.The results showed that the strains of wheat sharp eyespot in Yantai belonging to AGD,AG4 and AG5 three fusion groups,comprising 92.5%,5.0% and 2.5% respectively; the growth rate was between 5.33 to 13.0 mm?d1,dividing into three types: fast,medium and slow,comprising 55.0%,40.0% and 5.0% respectively.The 20 strains of AGD fusion group were randomly selected to determine their pathogenicity,and the disease index was between 16.67%~68.25%,which were divided into three grades: highly virulent,moderately virulent and weakly virulent strains; linear analysis revealed a positive correlation between growth rate and pathogenicity of Rhizoctonia strains in Yantai area.

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备注/Memo

收稿日期：2017-02-24基金项目：〖HTSS〗烟台市科技发展计划资助项目(2013NC327,2014NC107);国家现代农业产业技术体系资助项目(CARS3223)第一作者简介：于晓丽（1982-），女，山东省莱西市人，博士，农艺师，从事植物病原菌致病机理及防控技术研究。Email: yuxiaoli92jn@126.com。通讯作者：王英姿（1962-），女，山东省荣成市人，学士，研究员，从事植物病虫害防控技术研究。Email: ytnkyzbs@126.com。

更新日期/Last Update: 2017-08-09