[1]张 婧,孙婧欣,宋维富,等.春小麦新种质的分子细胞遗传学鉴定[J].黑龙江农业科学,2017,(03):1-7.[doi:10.11942/j.issn1002-2767.2017.03.0001]
　ZHANG Jing,SUN Jing-xin,SONG Wei-fu,et al.Molecular Cytogenetic Identification of New Germplasm on Spring Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(03):1-7.[doi:10.11942/j.issn1002-2767.2017.03.0001]
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春小麦新种质的分子细胞遗传学鉴定

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年03期 页码: 1-7 栏目: 出版日期: 2017-03-10
Title:
Molecular Cytogenetic Identification of New Germplasm on Spring Wheat
文章编号:
1002-2767（2017）03-0001-07
作者:
张 婧1; 孙婧欣1; 宋维富2; 杨雪峰2; 宋庆杰2; 李集临1; 张延明1
1.哈尔滨师范大学 生命科学与技术学院/黑龙江省分子细胞遗传与遗传育种重点实验室，黑龙江 哈尔滨150025；2.黑龙江省农业科学院 作物育种研究所，黑龙江 哈尔滨 150086
Author(s):
ZHANG Jing1; SUN Jing-xin1; SONG Wei-fu2; YANG Xue-feng2; SONG Qing-jie2; LI Ji-lin1; ZHANG Yan-ming1
1.Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province,College of Life Science and Technology,Harbin Normal University,Harbin,Heilongjiang 150025; 2.Crop Breeding Institute，Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086
关键词:
小麦; 种质资源; 远缘杂交; 基因组原位杂交
Keywords:
wheat;  germplasm resources;  distant hybridization;  GISH
分类号:
S512.1
DOI:
10.11942/j.issn1002-2767.2017.03.0001
文献标志码:
A
摘要:
远缘杂交能把亲缘关系较远的种、属中有用基因引入栽培种，改良现有品种。利用形态学和分子细胞遗传学研究方法，对远缘杂交创制的5份普通小麦新种质进行分析鉴定。结果表明：5份材料为春小麦，株系4-11和4-30-32突出表现为大穗、多分蘖，2015-2016年分蘖数为别为18~20和16~17个，熟期正常。二者的千粒重平均为38和36 g，籽粒蛋白质含量超过18%。株系6-30-31表现为矮秆（55 cm），密穗有芒，平均穗长7 cm，千粒重平均32.6 g。株系5-6和5-19，穗型似小偃麦，无芒，千粒重平均为33.7和36.4 g，籽粒蛋白质含量超过19%。5份材料根尖体细胞染色体数为42，减数分裂行为正常。分子标记和原位杂交检测表明，株系4-11和5-19带有中间偃麦草遗传成分，为小麦-中间偃麦草易位系，株系5-6为六倍体小偃麦，矮秆株系6-30-31带有E染色体组遗传成分。
Abstract:
The useful genes from a distant genetic relationship can be introduced into the cultivars by the distant hybridization for improving the available varieties.The five new common wheat germplasms were analyzed and identified by morphological and molecular cytogenetic methods.The results showed that five of the materials were spring wheat,and the character of lines 4-11 and 4-30-32 were large spike and multitillers.The number of tillers in 2015-2016 was 18~20 and 16~17.The average 1 000-grain weight was 38 g and 36 g,and the grain protein content was more than 18%.The character of line 6-30-31 was a dwarf (55 cm) and dense spike.The average spike length was 7 cm,and the average thousand seed weight was 32.6 g.Line 56 and 519 were spikelike trititrigia,no awn,and the average of 1000-grain weight was 33.7 g and 36.4 g,the protein content of kernel was more than 19%.The number of chromosomes was 42 in root tip cells of five materials,and the meiosis was normal.The results of molecular markers and GISH showed that line 4-11 and 5-19 had the genetic elements of Thinopyrum intermedium,which were wheatThinopyrum intermedium translocation lines.Line 5-6 were hexaploid trititrigia.The dwarf line 6-30-31 carried E genome genetic components.

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备注/Memo

收稿日期：2017-02-03
基金项目：国家重点研发计划资助项目(2016YFD0100102-16)；黑龙江省大学生创新创业训练计划重点资助项目（201610231024） 
第一作者简介：张婧（1992-），女，黑龙江省牡丹江市人，在读硕士，从事细胞遗传学研究。E-mail:350490922@qq.com。
通讯作者：张延明（1977-），男，博士，副教授，从事分子细胞遗传学研究。E-mail：blueright@163.com。

更新日期/Last Update: 2017-05-03