LIN Zhi-wei,CAO Qiang-wei,HUANG Zhi-yue,et al.Research on Enzyme Producing and Insecticidal Action of Beauveria bassiana Induced by Chitin[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(01):64-66.[doi:10.11942/j.issn1002-2767.2016.01.0064]
几丁质诱导球孢白僵菌产酶及杀虫作用研究
- Title:
- Research on Enzyme Producing and Insecticidal Action of Beauveria bassiana Induced by Chitin
- 文章编号:
- 1002-2767（2016）01-0064-03
- Keywords:
- Beauveria bassiana; chitinase; median lethal content(LC50)
- 分类号:
- Q939.96
- 文献标志码:
- A
- 摘要:
- 利用胶态几丁质诱导培养基液体发酵球孢白僵菌Bz4菌株，通过比色法测定发酵液中寡糖、几丁质酶的变化情况，并应用浸渍法测定其产生的孢子悬液对粘虫的致死能力，明确白僵菌Bz4菌株的应用价值。结果表明：球孢白僵菌Bz4菌株在供试的不同胶态几丁质浓度液体培养基中，均以培养5 d时产生的氨基寡糖量最高，而培养6 d时几丁质酶活力最大。利用几丁质培养基中制得的孢子悬液对粘虫幼虫具有较强的致死作用，致死中浓度约为1.04×105个?mL-1，其致死中时间为6~7 d。
- Abstract:
- Liquid fermentation of B.bassiana Bz4 strain induced by colloidal chitin was proceed,change of oligosaccharides and chitinase were measured during fermentation by colorimetric method,and the lethal capabilities of its spores suspension on armyworm was determined by the dipping method in order to utilize the B.bassiana Bz4 strain better.The results showed that the content of oligosaccharides was higher when cultivated B.bassiana Bz4 strain for 5 days,and the enzyme activity of chitinase was higher when cultivated B.bassiana Bz4 strain for 6 days under the condition of different colloidal chitin content.Lethal capability of spores suspension gained by colloidal chitin fermentation on armyworm was stronger,LC50 was 1.04×105?mL-1,LT50 time was 6 to 7 days.
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[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(01):72.[doi:10.11942/j.issn1002-2767.2019.12.0072]
备注/Memo
收稿日期：2015-11-16
基金项目：大学生创新创业资助项目；黑龙江农垦总局科技攻关资助项目
第一作者简介：林志伟（1970-），男，黑龙江省勃利县人，硕士，副教授，从事有害生物综合防治研究与教学工作。E-mail：408160466@qq.com。