ZHANG Yan,CHEN Jing,TIAN Jun-xiong,et al.Effect of Low P Stress on Maize Antioxidant System and Soluble Sugar Content[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(11):11-16.[doi:10.11942/j.issn1002-2767.2015.11.0011]
低磷胁迫对玉米抗氧化系统及可溶性糖含量阶段性变化的影响
- Title:
- Effect of Low P Stress on Maize Antioxidant System and Soluble Sugar Content
- 文章编号:
- 10022767（2015）11001106
- Keywords:
- maize; lowP stress; antioxidase; MDA; soluble sugar
- 分类号:
- S513
- 文献标志码:
- A
- 摘要:
- 为探讨低磷胁迫下玉米叶片抗氧化系统及可溶性糖含量的阶段变化特性与玉米耐低磷能力之间的关系，以2份高耐低磷（511、178）和2份对低磷胁迫高度敏感（492、9782）的玉米自交系为材料，采用河沙盆栽的方法，研究低磷胁迫对玉米叶片抗氧化系统及可溶糖含量阶段性变化的影响。结果表明：自交系的总体耐低磷能力为178＞511＞492＞9782。低磷胁迫下，玉米叶片的SOD活性、POD活性和可溶性糖含量先高于对照后低于对照，而MDA含量始终高于对照。4个自交系出苗期、拔节期、吐丝期和灌浆期相对SOD活性平均值分别为1.90、0.93、0.76、0.61，相对POD活性平均值分别为1.33、1.02、0.66、0.62，相对MDA含量平均值分别为1.18、1.77、1.52、1.60，相对可溶糖含量平均值分别为1.24、1.85、1.10、0.77。与敏感型自交系相比，耐低磷自交系的SOD和POD活性因低磷胁迫变化较小，MDA含量增加幅度也较小，而可溶糖含量在低磷胁迫早期增加幅度较大。
- Abstract:
- In order to explore the antioxidant system of maize under lowP stress and the relationship between of the soluble sugar content change and resistant ability against lowP， maize inbred lines 511(HT),178(HT),492(HS) and 9782(HS) were potted in sand to study the lowP stress effect on the changes of maize antioxidant system and soluble sugar content. The results showed that the tolerance to lowP stress was 178＞511＞492＞9782. Under lowP stress,SOD activity,POD activity and soluble sugar content were firstly higher than control and then lower,but the MDA content was higher than control all the time. The relative SOD activity at seedling,elongation,silking and filling stage averaged at 1.90,0.93,0.76 and 0.61 respectively. The average relative POD activity at the four stages were 1.33,1.02,0.66 and 0.62,average relative MDA content were 1.18,1.77,1.52 and 1.60,average relative soluble sugar content was 1.24,1.85,1.10 and 0.77,respectively. Compared with the HS lines,the SOD and POD activity of the HT lines was influenced less by lowP stress,MDA content increased less,but there was a larger increase of soluble sugar content at early stage.
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备注/Memo
基金项目：四川省教育厅自然科学重点资助项目（14ZA02-38） 第一作者简介：张燕（1979-），女，四川省珙县人，博士，副教授，从事作物抗逆机理研究。E-mail：243803088@qq.com。