[1]林花,金海强,姜银姬,等.灵芝多肽提取及冲剂制备工艺研究[J].黑龙江农业科学,2025,(03):54-60.[doi:10.11942/j.issn1002-2767.2025.03.0054]
　LIN Hua,JIN Haiqiang,JIANG Yinji,et al.Research on Extraction of Ganoderma lucidum Polypeptides and Preparation Process of Granules[J].HEILONGJIANG AGRICULTURAL SCIENCES,2025,(03):54-60.[doi:10.11942/j.issn1002-2767.2025.03.0054]
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灵芝多肽提取及冲剂制备工艺研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2025年03 页码: 54-60 栏目: 出版日期: 2025-03-10
Title:
Research on Extraction of Ganoderma lucidum Polypeptides and Preparation Process of Granules
文章编号:
9
作者:
林花1; 金海强1; 姜银姬1; 金美玉1; 王婷1; 金星爱1; 尹花2; 车成来1
（1.延边朝鲜族自治州农业科学院 农特产品加工研究所，吉林 延吉 133000； 2.龙井市人民医院，吉林 龙井 133400)
Author(s):
LIN Hua1; JIN Haiqiang1; JIANG Yinji1; JIN Meiyu1; WANG Ting1; JIN Xing′ai1;  YIN Hua2;  CHE Chenglai1
(1.Agricultural Products Processing Research Institute， Yanbian Korean Autonomous Prefecture Academy of Agricultural Sciences,Yanji 133000,China; 2.Longjing People′s Hospital, Longjing 133400, China)
关键词:
灵芝子实体; 活性多肽; 酶解法; 提取; 最佳条件
Keywords:
Ganoderma lucidum fruiting body;  active peptides; enzymatic hydrolysis method;  extraction;  optimal conditions
DOI:
10.11942/j.issn1002-2767.2025.03.0054
文献标志码:
A
摘要:
为探明超声波辅助酶解法在提取灵芝多肽时的最佳工艺参数，促进灵芝多肽的提取及相应产品的工厂化生产，以多肽提取量为参考指标，进行灵芝多肽的提取及灵芝多肽冲剂制备工艺研究，首先进行单因素试验，随后采用正交试验设计来优化结合超声波与酶法提取灵芝多肽的工艺条件，并进一步探究灵芝多肽冲剂的配方。结果表明，通过优化确定了灵芝多肽的最佳提取工艺参数，即料液比为1∶50，反应时间为2 h，反应温度为60 ℃，加酶量为6 000 U·g-1。此外，针对灵芝多肽冲剂的配方，也得出了最优比例，糊精占20%，淀粉占25%，柠檬酸占0.3%，蔗糖占6%。
Abstract:
In order to explore the optimal process parameters for ultrasonic assisted enzymatic hydrolysis in extracting Ganoderma lucidum peptides, promote the extraction of G.lucidum peptides and the industrial production of corresponding products, the extraction of G.lucidum peptides and the preparation process of G.lucidum peptide granules were studied with the peptide extraction amount as the reference index. Firstly, a single factor experiment was conducted, and then an orthogonal experimental design was used to optimize the process conditions for extracting G.lucidum peptides by combining ultrasonic and enzymatic methods, and further explore the formula of G.lucidum peptide granules.The results showed that through optimization, we determined the optimal extraction process parameters for G.lucidum peptides: a solid-liquid ratio of 1∶50, a reaction time of 2 hours, a reaction temperature of 60 ℃, and an enzyme dosage of 6 000 U·g-1. In addition, we have also determined the optimal ratio for the formula of G.lucidum peptide granules, with dextrin accounting for 20%, starch accounting for 25%, citric acid accounting for 0.3%, and sucrose accounting for 6%.

备注/Memo

收稿日期：2024-10-09

基金项目：延边州科技发展计划项目（2022GX30）。
第一作者：林花（1981-），女，硕士，副研究员，从事药食同源物质的有效成分提取及加工研究。E-mail:linhua0227@163.com。
通信作者：车成来（1971-），男，学士，副研究员，从事农特产品加工研究。E-mail:23124951@qq.com。

更新日期/Last Update: 2025-03-10