[1]刘畅,任爱华,冷春旭,等.秋子梨果实着色过程花青素含量及相关基因表达分析[J].黑龙江农业科学,2025,(03):39-45.[doi:10.11942/j.issn1002-2767.2025.03.0039]
　LIU Chang,REN Aihua,LENG Chunxu,et al.Analysis of Anthocyanin Content and Related Gene Expression During Fruit Coloring of Pyrus ussuriensis Maxim.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2025,(03):39-45.[doi:10.11942/j.issn1002-2767.2025.03.0039]
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秋子梨果实着色过程花青素含量及相关基因表达分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2025年03 页码: 39-45 栏目: 出版日期: 2025-03-10
Title:
Analysis of Anthocyanin Content and Related Gene Expression During Fruit Coloring of Pyrus ussuriensis Maxim.
文章编号:
7
作者:
刘畅1; 任爱华2; 冷春旭3; 顾广军1; 胡颖慧1; 杨悦1; 韩继龙2
（1.黑龙江省农业科学院 牡丹江分院/寒地果树育种栽培重点实验室，黑龙江 牡丹江 157000； 2.黑龙江省农业科学院 园艺分院，黑龙江 哈尔滨 150069； 3.黑龙江省农业科学院 生物技术研究所，黑龙江 哈尔滨 150023）
Author(s):
LIU Chang1;  REN Aihua2;  LENG Chunxu3;  GU Guangjun1;  HU Yinghui1;  YANG Yue1;  HAN Jilong2
(1.Mudanjiang Branch，Heilongjiang Academy of Agricultural Sciences/Key Laboratory of Breeding and Cultivation of Cold Region Fruit Trees, Mudanjiang 157000, China; 2.Horticulture Branch, Heilongjiang Academy of Agricultural Sciences, Harbin 150069, China; 3.Institute of Biotechnology, Heilongjiang Academy of Agricultural Sciences, Harbin 150023, China)
关键词:
秋子梨; ‘龙香’; ‘79-1’; 果实发育; 花青素; 基因表达
Keywords:
Pyrus ussuriensis Maxim.; Longxiang;  fruit development;  anthocyanin;  gene expression
DOI:
10.11942/j.issn1002-2767.2025.03.0039
文献标志码:
A
摘要:
为明确2个秋子梨果实不同发育期花青素含量，矢车菊素-3-O-葡萄糖苷和矢车菊素-3-O-半乳糖苷两个花青素主要成分积累的变化趋势以及花青素相关基因表达水平。以‘龙香’和‘79-1’秋子梨5个发育期果实为材料，分别测定花青素及其两个主要成分的含量，分析花青素及其主成分与相关基因之间的联系。结果表明，2个秋子梨品种果实发育过程中，‘79-1’花青素含量高于‘龙香’，花后100 d花青素含量达到最高，果实发育期花青素2个主要成分含量整体呈先升高后下降的趋势，同样在花后100 d含量达到峰值。花青素合成基因PbMYB108、PbMYC2、PbbHLH130和PbbHLH62的相对表达量在果实的整个发育期均呈先上升后下降的趋势，与花青素两个主要成分的变化趋势相同。除PbMYC2外，PbbHLH130、PbbHLH62和PbMYB108转录调控基因在花后40 d时秋子梨‘79-1’的基因表达量均低于‘龙香’。相关性分析结果显示PbMYC2的表达水平与花青素含量之间无明显的相关性，PbMYB108和PbbHLH62与花青素含量的相关系数在两个品种中均大于0.5，推测它们对花青素的合成起重要作用。
Abstract:
In order to clarify the anthocyanin content in two Pyrus ussuriensis Maxim. fruits at different developmental stages, the accumulation trends of the two main components, cyanidin-3-O-glucoside and cyanidin-3-O-galactoside, as well as the expression levels of anthocyanin-related genes. In this study, the contents of anthocyanins and two main components of 5 growing fruits of Longxiang and 79-1 Pyrus ussuriensis Maxim. were determined, and the relationship between anthocyanins and main components and related genes were analyzed. The results showed that the anthocyanin content of 79-1 was higher than that of Longxiang during the fruit development of the two Pyrus ussuriensis Maxim. varieties, and the anthocyanin content reached the highest 100 days after flowering The content of two main anthocyanins in the fruit showed an overall trend of first increasing and then decreasing during the fruit development stage, and content reached a peak at 100 days after flowering. The relative expression of anthocyanin synthesis genes PbMYB108, PbMYC2, PbbHLH130 and PbbHLH62 showed a trend of first rising and then falling throughout the whole development period of fruit, which was the same as that of the two main components of anthocyanin. In addition to PbMYC2, the expression levels of three other transcriptional regulatory genes, PbbHLH130, PbbH62, and PbMYB108, were lower in 79-1 than in Longxiang at 40 days after flowering The results of correlation analysis showed that the expression level of PbMYC2 was not significantly correlated with the anthocyanin content, while the coefficients between PbMYB108 and PbbHLH62 and anthocyanin content were greater than 0.5 in both varieties, that they play an important role in anthocyanin synthesis.

相似文献/References:

[1]刘春光,陶靓,张武杰,等.两个秋子梨果实品质分析[J].黑龙江农业科学,2024,(05):49.[doi:10.11942/j.issn1002-2767.2024.05.0049]
　LIU Chunguang,TAO Liang,ZHANG Wujie,et al.Fruit Quality Analysis of Two Varieties of Pyrus ussuriensis Maxim.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(03):49.[doi:10.11942/j.issn1002-2767.2024.05.0049]

备注/Memo

收稿日期：2024-10-31

基金项目：黑龙江省科研业务费（CZKYF2022-1-B030）。
第一作者：刘畅（1984-），男，博士，副研究员，从事寒地果树种质资源收集及新品种选育研究。E-mail：changchang_3000@163.com。
通信作者：顾广军（1980-），男，硕士，副研究员，从事寒地果树育种研究。E-mail：ggj-163@163.com。

更新日期/Last Update: 2025-03-10