WANG Jiangxu.Mapping and Analysis of Rice Flag Leaf Width Gene Based on GWAS[J].HEILONGJIANG AGRICULTURAL SCIENCES,2023,(02):1-5.[doi:10.11942/j.issn1002-2767.2023.02.0001]
基于关联分析的水稻剑叶宽基因定位与分析
- Title:
- Mapping and Analysis of Rice Flag Leaf Width Gene Based on GWAS
- 文章编号:
- 1
- Keywords:
- rice; leaf shape; flag leaf width; GWAS; main QTLs
- 文献标志码:
- A
- 摘要:
- 叶形是影响粳稻光合作用和碳水化合物积累的重要因素之一，而叶宽是决定水稻叶形的核心性状之一。挖掘和利用新的叶宽相关基因/QTL可以进一步丰富分子育种理论，提升叶宽性状遗传改良的效率。本研究利用295份粳稻品种的自然群体，于2020年和2021年对各品种的剑叶宽进行考察。结合高通量重测序获得的788 396个高质量多态性SNP，对粳稻剑叶宽相关性状进行全基因组关联分析(GWAS)，共检测到45个关联SNPs，分布在水稻的12条染色体上，表型贡献率范围为861%~1576%。其中，qFL10在两年间被重复检测到，定位区间在10.11 Mb~10.23 Mb，此区段内包含15个基因。
- Abstract:
- Leaf shape is one of the important factors affecting photosynthesis and carbohydrate accumulation in rice (Oryza sativa Geng Group), while leaf width is one of the core traits determining leaf shape in rice. Mining and using new leaf width related genes/QTLs can further enrich molecular breeding theory and improve the effect of genetic improvement of leaf width traits. In this study, 295 natural populations of rice (Oryza sativa Geng Group) varieties were used to investigate the flag leaf width of each variety in 2020 and 2021. Combined with 788 396 high-quality polymorphic SNPs obtained by high-throughput re-sequence, GWAS was performed on the correlation of flag leaf width in rice. A total of 45 associated SNPs were detected, distributed on 12 chromosomes of rice, and the phenotypic contribution rate ranged from 8.61% to 15.76%. Among them, qFL10 was detected repeatedly in two years, and the localization interval was 1011 Mb-10.23 Mb, which contained 15 genes.
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备注/Memo
基金项目：黑龙江省省属科研院所科研业务费（CZKYF2022-1-B011）；黑龙江省农业科学院院级课题（2021YYYF037）；高端外国专家引进项目（G2022011015）