CHEN Shu,CAI Shi-ping,JIANG Wei,et al.Physiological Responses of Two Mulberry Species to Salt Stress[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(12):24-28.[doi:10.11942/j.issn1002-2767.2022.12.0024]
两种桑树对盐胁迫的生理响应
- Title:
- Physiological Responses of Two Mulberry Species to Salt Stress
- Keywords:
- Morus alba L.; root; salt-tolerance; antioxidant enzyme; malondialdehyde
- 文献标志码:
- A
- 摘要:
- 为了探究不同品种桑树根部对盐胁迫的生理响应机制，本研究以桂桑和蛋白桑为试验材料，研究3个不同NaCl浓度处理下两个品种桑树根部的生理生化指标变化。结果表明，与正常生长组（CK）相比，超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性均随着盐浓度的增加呈现先增加后降低的趋势，且当浓度为50 mmol·L-1时，两者酶活性都达到最高值；过氧化氢酶(CAT)活性随着盐浓度的增加呈现出持续上升的变化趋势，在盐分浓度从0 mmol·L-1升高到50 mmol·L-1阶段，其活性变化趋势比50~100 mmol·L-1更加明显；盐胁迫后，两种桑树幼苗根部的丙二醛(MDA)含量均低于对照组，且当盐浓度达到100 mmol·L-1时，桂桑根部MDA含量低于蛋白桑；桂桑根部的过氧化氢(H2O2)含量呈现下降的趋势，蛋白桑根部的过氧化氢(H2O2)含量呈现先上升后下降的变化趋势。综合两个桑树品种根系的生理生化指标结果，可初步推断桂桑的抗盐性强于蛋白桑。
- Abstract:
- In order to explore the physiological response mechanism of mulberry roots of different varieties to salt stress， this study took Guisang and Danbaisang as experimental materials to study the changes of physiological and biochemical indexes of mulberry roots of two varieties under different NaCl concentrations. The results of setting three concentration gradients showed that compared with the normal growth group (CK)， the activities of superoxide dismutase (SOD) and peroxidase (POD) increased first and then decreased with the increase of salt concentration in 50 and 100 mmol·L-1 NaCl treatments，and when the concentration was 50 mmol·L-1， the enzymatic activities of both reached the highest value; Catalase (CAT) activity showed a continuous upward trend with the increase of salt concentration.At the stage of salt concentration rising from 0 mmol·L-1 to 50 mmol·L-1， the change trend of CAT activity was more obvious than that of 50 mmol·L-1 to 100 mmol·L-1; After salt stress， the content of malondialdehyde (MDA) in the roots of two mulberry seedlings was lower than that of the control group，and when the salt concentration reached 100 mmol·L-1， the content of MDA in the roots of guisang was lower than that of proteinsang; The content of hydrogen peroxide (H2O2) in the roots of Guisang showed a downward trend， and the content of hydrogen peroxide(H2O2) in the roots of Danbaisang showed a change trend of increasing first and then decreasing. Based on the results of physiological and biochemical indexes of the roots of two mulberry varieties， it can be preliminarily concluded that the salt resistance of Guisang is stronger than that of Danbaisang.
参考文献/References:
［1］秦俭，何宁佳，黄先智，等.桑树生态产业与蚕丝业的发展[J].蚕业科学，2010，36(6):984-989.
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备注/Memo
收稿日期：2022-09-10