LIU Li-bin,XIE Yan-ping,YANG Wei,et al.Effects of Atrazine Stress on Physiological and Biochemical Indexes of Setaria italica L.with Different Atrazine Resistance[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(03):43-48.[doi:10.11942/j.issn1002-2767.2022.03.0043]
莠去津胁迫对不同抗性谷子生理生化指标的影响
- Title:
- Effects of Atrazine Stress on Physiological and Biochemical Indexes of Setaria italica L.with Different Atrazine Resistance
- Keywords:
- Setaria italica L.; atrazine; physiological and biochemical indexes; resistant variety; sensitive variety
- 文献标志码:
- A
- 摘要:
- 为进一步验证萌芽期筛选的抗/感莠去津谷子品种公矮2号和龙谷31的抗耐性,本试验观察了莠去津胁迫下谷子幼苗生长情况,并测定了莠去津胁迫下谷子幼苗叶片和根的抗氧化保护酶活性(POD、SOD)、可溶性蛋白、脯氨酸、丙二醛(MDA)含量的变化。结果表明:莠去津胁迫下,敏感品种(龙谷31)和抗性品种(公矮2号)幼苗生长均受到抑制;其他各项生理生化指标含量均与对照相比具有显著差异,其中龙谷31 叶片中MDA和渗透调节物质含量增量均高于公矮2号,而保护酶活性则低于公矮2号。综合分析,莠去津胁迫下,抗性品种公矮2号能够维持较高的抗氧化保护酶活性,叶片积累较少的MDA,可以较好地缓解莠去津对谷子幼苗的伤害,使植物体内维持正常的各种代谢活动。
- Abstract:
- In order to further verify the atrazine resistant/susceptible millet varieties Gongai 2 and Longgu 31,the growth of seedling was observed,and the activity of antioxidant protective enzyme activities (POD and SOD) and the contents of soluble protein,proline and malondialdehyde (MDA) in leaves and roots of millet seedlings under atrazine stress were determined in this study.The results showed that under atrazine stress,the inhibition degree of seedling growth indexes of sensitive variety (Longgu 31) and resistant variety (Gongai 2)were all great; the contents of other physiological and biochemical indexes were significantly different from the control,in which the increment of MDA and osmoregulation substances in Longgu 31 leaves were higher than Gongai 2,while the activity of protective enzyme was lower than Gongai 2.Comprehensive analysis showed that under atrazine stress,resistant variety Gongai 2 could maintain high antioxidant protective enzyme activity and accumulate less MDA in leaves,which could better alleviate the damage of atrazine to millet seedlings,so as to maintain normal metabolic activities in plants.
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备注/Memo
收稿日期:2021-12-10 基金项目:黑龙江八一农垦大学创新创业项目(XC2019018);黑龙江省博士后科研启动项目(LBH-Q18101);黑龙江省重点研发项目(GA21B009-5)。 第一作者:刘丽滨(1998-),男,硕士研究生,从事作物遗传育种研究。E-mail:1151542823@qq.com。 通信作者:孙丽芳(1981-),女,博士,副教授,从事作物遗传育种研究。E-mail:sunlifang2013@126.com。