[1]陈国峰,尤宏梅,王贵江,等.高效液相色谱法同时测定工业大麻中四种大麻素的方法研究[J].黑龙江农业科学,2021,(11):60-64.[doi:10.11942/j.issn1002-2767.2021.11.0060]
　CHEN Guo-feng,YOU Hong-mei,WANG Gui-jiang,et al.Simultaneous Determination of Four Cannabinoids in Industrial Hemp by High Performance Liquid Chromatography[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(11):60-64.[doi:10.11942/j.issn1002-2767.2021.11.0060]
点击复制

高效液相色谱法同时测定工业大麻中四种大麻素的方法研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2021年11 页码: 60-64 栏目: 出版日期: 2021-11-10
Title:
Simultaneous Determination of Four Cannabinoids in Industrial Hemp by High Performance Liquid Chromatography
作者:
陈国峰1; 尤宏梅1; 王贵江2; 张晓波1; 刘 峰1; 董见南1; 黄文功1; 任红波1
1.黑龙江省农业科学院 农产品质量安全研究所，黑龙江 哈尔滨 150086；2.黑龙江省农业科学院，黑龙江 哈尔滨 150086
Author(s):
CHEN Guo-feng1; YOU Hong-mei1; WANG Gui-jiang2; ZHANG Xiaobo1; LIU Feng1; DONG Jian-nan1; HUANG Wengong1; REN Hong-bo1
1.Safety and Quality Institute of Agricultural Products,Heilongjiang Academy of Agricultural Sciences，Harbin 150086,China; 2.Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China
关键词:
大麻素; 高效液相色谱; 工业大麻; 大麻二酚; 四氢大麻酚
Keywords:
cannabinoids;  high performance liquid chromatography;  industrial hemp; cannabidiol(CBD); tetrahydrocannabinol(THC)
DOI:
10.11942/j.issn1002-2767.2021.11.0060
文献标志码:
A
摘要:
为了应对黑龙江省工业大麻的快速发展所带来的工业大麻花、叶、茎秆、种子等的大量检测需求，本文建立了工业大麻花、叶、茎秆和种子中大麻二酚酸（CBDA）、大麻二酚（CBD）、四氢大麻酚酸（THCAA）和四氢大麻酚（THC）同时测定的高效液相色谱法。工业大麻花、叶、茎秆和种子中的4种大麻素经20 mL的90∶10（V∶V）甲醇正己烷溶液分两次振荡提取后离心，收集全部上清液混合后，过滤。提取液采用C18色谱柱分离，可变波长紫外检测器检测，基质加标法定量。结果表明：大麻二酚、大麻二酚酸、四氢大麻酚和四氢大麻酚酸4种大麻素在0.5~25.0 mg·L-1之间线性关系良好，相关系数R2均大于0.999 4，4种大麻素的提取回收率为89.6%~99.7%，相对标准偏差为1.5%~4.8%，检出限为0.1 mg·kg-1。该方法具有快速、操作简单、灵敏度高等优点，可满足工业大麻中大麻二酚、大麻二酚酸、四氢大麻酚和四氢大麻酚酸的检测要求。
Abstract:
In order to meet the large detection needs of industrial hemp flowers,leaves,stems and seeds brought by the rapid development of industrial hemp in Heilongjiang Province,the method of simultaneous determination of the cannabidiol acid(CBDA),cannabidiol(CBD),tetrahydrocannabinolic acid(THCAA)and tetrahydrocannabinol(THC)by high performance liquid chromatography were established.The four cannabinoids in industrial hemp flowers and leafs,stems,seeds were extracted with 20 mL methanolhexane solution of 90∶10 (V∶V) twice by shaking,then centrifuged,and all the supernatants were collected and mixed.The extract solution was separated by a C18 chromatographic column,detected by a variable wavelength ultraviolet detector (UVD),and quantified by matrix addition.The results showed that the four cannabidiols had a good linear relationship between 0.5-25.0 mg·L-1,and the correlation coefficient R2 was greater than 0.999 4.The recovery of cannabinoids were between 89.6% and 99.7%,the relative standard deviation(RSD)was ranged from 1.5% to 4.8%,and the LOQ was 0.1 mg·kg1.The method has the advantages of rapidness,simple operation,high sensitivity,etc.,and can meet the detection requirements of CBDA,CBD,THC and THCA in industrial hemp.

参考文献/References:

［1］张景,孙武兴,王曙宾,等.工业大麻提取物中四氢大麻酚定量检测方法研究[J].绿色科技,2020(12): 216-218,220.

[2]胡赠彬.实验室检测工业大麻及相关产品的安全管理[J].食品安全质量检测学报,2020,11(18):6311-6317.
[3]孙冬梅,兰韬,云振宇,等.火麻油中五种大麻素的HPLC同时检测[J].食品工业科技，2021，42（22）：263-269.
[4]吴鹏,杨丽君,刘东麟,等.云南、黑龙江两省工业大麻种植加工的合法化对吉林省的启示[J].中国麻业科学,2021,
43(2):88-96.
[5]刘胜贵,马海悦,李智高,等.HPLC法测定工业大麻花叶中的CBD和THC的含量[J].云南化工,2020,47(5):62-64.
[6]张晓艳,王晓楠,曹焜,等.碱性盐胁迫对3个国外引进工业大麻品种萌发特性的影响及评价[J].种子,2021,40(6):58-63.
[7]严江涛,常丽,张江江,等.工业大麻中大麻二酚提取工艺优化和生物活性分析[J].中国麻业科学,2020,42(2): 61-69.
[8]孙孔春,陈兴龙,杨璨瑜,等.不同条件下工业大麻中大麻二酚含量变化[J].昆明医科大学学报，2020,41(5)：23-28.
[9]唐慧娟,臧巩固,程超华,等.工业大麻产量和品质性状的对应分析[J].作物杂志,2018(2): 20-24.
[10]周莹,陈念念,韩丽,等.国内外工业大麻食品法律法规及大麻素检测方法研究进展[J].食品安全质量检测学报,2019,10(23): 7959-7966.
[11]张晓艳,曹焜,韩承伟,等.3个国外引进工业大麻品种在轻、中度盐碱土生长发育特性的研究[J/OL].东北农业科学:17[20210517].http://kns.cnki.net/kcms/detail/22.1376.S.20210517.1226.008.html.
[12]张庆滢,郭蓉,许艳萍,等.不同类型地膜覆盖对工业大麻生长和产量的影响[J].中国麻业科学,2020,42(5):239-243.
[13]孙哲,王金娥,乔永刚.工业大麻发育早期雌雄株鉴定方法的研究[J].农业与技术,2021，41(14):70-71.
[14]许艳萍,杨明,郭鸿彦,等.5个工业大麻品种对5种重金属污染土壤的修复潜力[J].作物学报,2020,46(12):1970-1978.
[15]孟沛鑫,赵毅,贾明宏.ICPMS法测定不同品种工业大麻秆中 5 种重金属元素[J].安徽农业科学,2021,49(9):177-179.
[16]解国庆,董清山,范书华,等.八个不同基因型工业大麻（药用）在黑龙江省东南部的适应性评价[J].黑龙江农业科学,2021(6):9-12.
[17]黄峰华.黑龙江省麻类产业现状及发展建议[J].辽宁农业科学,2017(5):70-71.
[18]张明,张利国,房郁妍,等.黑龙江省工业大麻产业现状及未来发展建议[J].黑龙江农业科学,2020(12):123-125.
[19]赵越,王晓楠,孙宇峰，等.工业大麻纤维产量、品质影响因素及纤维发育相关基因研究进展[J].中国麻业科学,2021,43(3):155-160.
[20]王明明,景尚友,李彩华,等.浅析黑龙江省药用大麻品种选育策略[J].现代化农业,2020（8）:3637.[21]HDENER M,KNIG S,WEINMANN W.Quantitative determination of CBD and THC and their acid precursors in confiscated cannabis samples by HPLCDAD[J].Forensic Science International,2019,299:142-150.

相似文献/References:

[1]宋伟丰.蛇床子中蛇床子素的提取及检测方法的研究[J].黑龙江农业科学,2013,(02):104.
　SONG Wei-feng.Study on Abstracting and Detecting Method of Osthol in Frutus cnidii[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(11):104.
[2]郭春景,孙长华,王玉芝.快速溶剂萃取-高效液相色谱法测定莎稗磷残留[J].黑龙江农业科学,2011,(07):67.
　GUO Chun-jing,SUN Chang-hua,WANG Yu-zhi.Residue Determination of Anilofos in Soil and Rice by Rapid Pressure Organic Solvents Extraction-High Performance Liquid Chromatography[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(11):67.
[3]孙延芳,梁宗锁,杨开宝,等.高效液相色谱法分析酸枣中的有机酸和维生素C[J].黑龙江农业科学,2011,(08):80.
　SUN Yan-fang,LIANG Zong-suo,YANG Kai-bao,et al.Analysis of Organic Acids and Vc in Sour Jujube byHigh Performance Liquid Chromatography[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(11):80.
[4]王若丁,王 超,孟凡娟,等.工布乌头中三种生物碱HPLC法测定优化[J].黑龙江农业科学,2014,(01):106.
　WANG Ruo-ding,WANG Chao,MENG Fan-juan,et al.The Optimization of HPLC Determination for Three Kinds of Alkaloids in Aconitum kongboense L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(11):106.
[5]蒋冰清,罗盛东.HPLC法测定滇桂艾纳香中原儿茶酸和原儿茶醛的含量[J].黑龙江农业科学,2015,(03):120.[doi:10.11942/j.issn1002-2767.2015.03.0120]
　JIANG Bing-qing,LUO Sheng-dong.Determination of Protocatechuic Acid and Protocatechuic Aldehyde in Blumeae ripariaeby HLPC[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(11):120.[doi:10.11942/j.issn1002-2767.2015.03.0120]
[6]张鑫喆,赵琳琳,徐方旭,等.HPLC不同流动相测定蛹虫草中虫草素和腺苷的比较[J].黑龙江农业科学,2018,(04):130.[doi:10.11942/j.issn1002-2767.2018.04.0130]
　ZHANG Xin-zhe,ZHAO Lin-lin,XU Fang-xu,et al.Comparison of Cordycepin and Adenosine in Cordyceps Militaris with Different Mobile Phases by HPLC[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(11):130.[doi:10.11942/j.issn1002-2767.2018.04.0130]
[7]牛庭莉,张树权,韩喜财,等.大麻雌化处理方法的研究进展[J].黑龙江农业科学,2022,(01):100.[doi:DOI:10.11942/j.issn1002-2767.2022.01.0100]
　NIU Ting-li,ZHANG Shu-quan,HAN Xi-cai,et al.Research Progress on Female Treatment of Cannabis[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(11):100.[doi:DOI:10.11942/j.issn1002-2767.2022.01.0100]
[8]章厉劼,廖欢,栾庆祥,等.HPLC法测定兽用银翘散中绿原酸和连翘苷含量[J].黑龙江农业科学,2022,(10):83.[doi:10.11942/j.issn1002-2767.2022.10.0083]
　ZHANG Li-jie,LIAO Huan,LUAN Qing-xiang,et al.Determination of Chlorogenic Acid and Forsythin in Veterinary Yinqiao Powder by HPLC[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(11):83.[doi:10.11942/j.issn1002-2767.2022.10.0083]

备注/Memo

收稿日期：2021-09-09

基金项目：黑龙江省农业科学院“农业科技创新跨越工程”专项：多种酚和酚酸类物质的检测研究与标准制定（MLCX20-4）。
第一作者：陈国峰（1983-），男，博士，副研究员，从事工业大麻中烯、醇、酚、酮类物质的检测研究。E-mail:hljjiance@163.com。

更新日期/Last Update: 2021-12-05