[1]周菲,王文军,刘岩,等.向日葵HafabZ基因的生物信息学和表达分析[J].黑龙江农业科学,2021,(07):11-14.[doi:10.11942/j.issn1002-2767.2021.07.0011]
　ZHOU Fei,WANG Wen-jun,LIU Yan,et al.Bioinformatics and Expression Analysis of HafabZ Gene in Sunflower[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(07):11-14.[doi:10.11942/j.issn1002-2767.2021.07.0011]
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向日葵HafabZ基因的生物信息学和表达分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2021年07 页码: 11-14 栏目: 出版日期: 2021-07-10
Title:
Bioinformatics and Expression Analysis of HafabZ Gene in Sunflower
作者:
周菲1; 2; 王文军2; 刘岩2; 黄绪堂1; 2; 吴立仁2; 王静2; 姚玉波2; 李明东3
1.黑龙江省农业科学院 博士后科研工作站，黑龙江 哈尔滨 150086；2.黑龙江省农业科学院经济作物研究所，黑龙江 哈尔滨 150086；3.黑龙江省五大连池农场，黑龙江 五大连池 164100
Author(s):
ZHOU Fei1; 2; WANG Wen-jun2; LIU Yan2; HUANG Xu-tang1; 2 WU Li-ren2; WANG Jing2; YAO Yu-bo2; LI Ming-dong3
1.Post Doctoral Research Station，Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China; 2.Industrial Crops Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China；3.Wudalianchi Farm of Heilongjiang Province,Wudalianchi 164100,China
关键词:
向日葵; 油酸; HafabZ; 生物信息学; 表达分析
Keywords:
sunflower;  oleic acid;  HafabZ;  bioinformatics;  expression anaylsis
DOI:
10.11942/j.issn1002-2767.2021.07.0011
文献标志码:
A
摘要:
为进一步了解向日葵HafabZ基因的表达特性及功能，通过数据库检索（NCBI）及荧光定量PCR等方法对向日葵HafabZ编码蛋白进行生物信息学和表达模式分析。结果表明：预测HafabZ编码蛋白为酸性且为亲水性蛋白，二级结构主要为无规则卷曲，预测亚细胞定位于叶绿体；系统进化分析发现，HafabZ与薇甘菊fabZ进化关系最近，序列相似性为83％；HafabZ基因表达分析结果表明，在向日葵“L-1-OL-1”开花后7 d，HafabZ在向日葵中表达量从高到低排序为：种子>舌状花>管状花>叶>茎>根。在种子发育不同时期，HafabZ在高、低油酸向日葵材料发育前期种子中表达量均较高，均在开花后17 d表达量达到最高，之后均明显下调，在开花后22和27 d，高油酸向日葵中表达量明显低于低油酸向日葵，表明这段发育时期HafabZ在高、低油酸向日葵种子中可能存在不同的表达调控机制。
Abstract:
In order to further understand the expression characteristics and functions of HafabZ gene in sunflower,the bioinformatics and expression pattern of HafabZ encoded protein in sunflower were analyzed by database retrieval(NCBI) and fluorescence quantitative PCR.The results showed that the protein was predicted to be acidic and hydrophilic,and its secondary structure was mainly irregular curl; phylogenetic analysis showed that HafabZ had the closest evolutionary relationship with Mikania micrantha L.fabZ,and the sequence similarity was 83%.The results of HafabZ gene expression analysis showed that the order of HafabZ expression from high to low was seed>tongue floret>tube floret>leaf>stem>root on the 7th day after flowering of sunflower “L-1-OL-1”.At different stages of seed development,the expression levels of HafabZ in high and low oleic acid sunflower materials were higher at the early stage of seed development,and reached the highest level at 17 days after anthesis,and then decreased significantly.At 22 and 27 days after anthesis,the expression levels of HafabZ in high oleic acid sunflower materials were significantly lower than that in low oleic acid sunflower materials.These results suggest that there may be different regulation mechanisms of HafabZ expression in high and low oleic acid sunflower seeds during this developmental period.

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备注/Memo

收稿日期：2021-04-21

基金项目：黑龙江省农业科学院院级科研项目（2020FJZX005）；财政部和农业农村部：国家现代农业产业技术体系；黑龙江省农业科学院“农业科技创新跨越工程”专项（HNK2019CX14）；全国基层农技推广体系改革与建设补助项目（KYBG-05WDL-2020001）。
第一作者：周菲（1984-），女，博士，助理研究员，从事向日葵品质育种研究。E-mail:zhoufei66666@163.com。
通信作者：黄绪堂（1964-），男，硕士，研究员，从事向日葵遗传育种研究。E-mail：huangxutang@126.com。

更新日期/Last Update: 2021-07-21