[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(06):119-124.[doi:10.11942/j.issn1002-2767.2021.06.0119]
羊草幼穗组织培养与再生体系建立的研究
- 文献标志码:
- A
- 摘要:
- 为了优化羊草组织培养与再生体系，本试验以6个羊草[Leymus chinensis(Trin.)Tzvel.]新品系的幼穗为外植体，使用添加不同植物激素的MS培养基对羊草幼穗进行愈伤组织诱导、继代、分化等培养，建立高效再生体系。结果表明：诱导培养基中添加2，4-D激素浓度为5.0 mg?L-1时，6个羊草新品系幼穗的诱导率均较高，其中Lc-6出愈率最高，为87.5%，其次是Lc-4，诱导率为81.5%；6个品系愈伤组织通过固-液-固（MS2-MSL2-MS2)交替继代培养，愈伤组织结构与状态得到明显改善，愈伤由疏松变得致密，褐化现象明显减少，愈伤变为乳白色颗粒状，Lc-6和Lc-4的愈伤状态优于其他品系；6个品系愈伤组织在BNKT分化培养基上的分化率显著高于其他2种培养基（P < 0.05），Lc-6分化率最高，为41.25%；6个品系移栽后组培苗成活率均为100%。
相似文献/References:
[1]孙岩,张宏纪,王广金,等.航天处理对小麦组织培养效果的研究[J].黑龙江农业科学,2014,(11):1.
SUN Yan,ZHANG Hong-ji,WANG Guang-jin,et al.Study on the Effect of Space Treatment on Wheat Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(06):1.
[2]王碧才,丰 锋.文心兰不定芽生根培养基配方的优化与移栽基质筛选[J].黑龙江农业科学,2014,(11):12.
WANG Bi-cai,FENG Feng.Optimization of Rooting Medium and Screening of Transplanting Medium of Oncidium[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(06):12.
[3]曹天旭,宿肇辉,韩超慧.铁皮石斛组培快繁技术的研究[J].黑龙江农业科学,2014,(05):30.
CAO Tian-xu,SU Zhao-hui,HAN Chao-hui.Study on Tissue Culture and Rapid Propagation of Dendrobium candidum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(06):30.
[4]唐文杰,王玉,周泉澄,等.马齿苋组培与耐盐筛选[J].黑龙江农业科学,2013,(03):7.
TANG Wen-jie,WANG Yu,ZHOU Quan-cheng,et al.Tissue Culture and Salt Resistance Selection of Portulaca oleracea L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(06):7.
[5]尹相博,于咏梅,于立芝.红树莓组织培养研究进展[J].黑龙江农业科学,2013,(02):140.
YIN Xiang-bo,YU Yong-mei,YU Li-zhi.Review of Progress in Tissue Culture of Red Raspberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(06):140.
[6]陈荣珠,沈应柏,罗晓芳,等.连香树组织培养及植株再生体系的初步研究[J].黑龙江农业科学,2012,(01):16.
CHEN Rong-zhu,SHEN Ying-bai,LUO Xiao-fang,et al.Research on Tissue Culture and Plant Regeneration of Cercidiphyllum japonicum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(06):16.
[7]曹羽.香花槐组织培养研究现状[J].黑龙江农业科学,2012,(04):142.
CAO Yu.Research Advance in Tissue Culture of Robinia pseudoacaia ‘Idaho’[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(06):142.
[8]张瑜,柏彦伸,孙靖靓,等.活血丹子叶无性系建立的研究[J].黑龙江农业科学,2012,(11):21.
ZHANG Yu,BAI Yan-shen,SUN Jing-liang,et al.Study on Establishment of Clone for Giechoma longituba[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(06):21.
[9]时群,何贵整,陈乃明,等.木薯产业化组培育苗技术研究[J].黑龙江农业科学,2012,(11):25.
SHI Qun,HE Gui-zheng,CHEN Nai-ming,et al.Study on Industrialization Tissue CultureSeedlings Technology of Cassava[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(06):25.
[10]张元国,李 芳,包艳存,等.芦笋全雄品种WF-8茎尖组培快繁技术研究[J].黑龙江农业科学,2012,(12):21.
ZHANG Yuan-guo,LI Fang,BAO Yan-cun,et al.Study on Tissus Culture Rapid Reproduction Technology of Stem Tip of All Male Asparagus Cultivar WF-8[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(06):21.
备注/Memo
收稿日期：2021-03-24