DU Xiang-ying,XIE Ying-yuan,LIAO Fei-yang,et al.Study on the Selection and Initiation of Casuarina equisetifolia Explants[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(01):104-106.[doi:10.11942/j.issn1002-2767.2020.01.0104]
木麻黄外植体选择与启动研究
- Title:
- Study on the Selection and Initiation of Casuarina equisetifolia Explants
- Keywords:
- Casuarina equisetifolia; explants; sterilization; anti-browning; tissue culture
- 文献标志码:
- A
- 摘要:
- 为探索木麻黄优良无性系品种抗性退化问题,对木麻黄优良无性系外植体启动进行组织培养试验,通过9组方案进行组合试验。结果表明:木麻黄以木质化外植体,选择用70%乙醇+0.3%新洁尔灭+3%次氯酸钠+0.5%升汞的灭菌方案,能达到最佳的少毒杀、灭菌、少褐化、高成活率等的效果。
- Abstract:
- In order to explore the problem of resistance degradation of superior clones of Casuarina equisetifolia,the tissue culture experiment was carried out on the explants of superior clones of Casuarina equisetifolia through 9 groups of schemes.The results showed that the best sterilization scheme was 70% ethanol + 0.3%Bromogeramine+3%sodium hypochlorite+0.5% mercuric chloride,which could achieve the effects of less toxicity,sterilization,less browning and high survival rate.
参考文献/References:
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备注/Memo
收稿日期:2019-08-01第一作者:杜翔鹰(1986-),男,林业工程师,从事植物组织培养研究。E-mail:dutstudent@126.com。