[1]杨帆,刘春来,王爽,等.一株虫生真菌的分离鉴定及生防潜力研究[J].黑龙江农业科学,2019,(12):72-77.[doi:10.11942/j.issn1002-2767.2019.12.0072]
　[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(12):72-77.[doi:10.11942/j.issn1002-2767.2019.12.0072]
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一株虫生真菌的分离鉴定及生防潜力研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年12期 页码: 72-77 栏目: 出版日期: 2019-12-10
作者:
杨帆; 刘春来; 王爽; 刘亮; 蒋希峰; 李新民
黑龙江省农业科学院 植物保护研究所/农业部哈尔滨作物有害生物科学观测实验站，黑龙江 哈尔滨 150086
关键词:
球孢白僵菌; 生物学特性; 致病性; 抑菌作用
DOI:
10.11942/j.issn1002-2767.2019.12.0072
文献标志码:
A
摘要:
为挖掘虫生真菌资源，获得高效生防菌，本文利用大蜡螟诱集法从东北地区不同类型土样中筛选虫生真菌，其中选取了一株虫生真菌FLNXY5菌株，经形态学及18s rDNA ITS序列分析鉴定为球孢白僵菌Beauveria bassiana（KY419577）。本研究对FLNXY5菌株生物学特性、对小菜蛾Plutella xylostella 2龄幼虫的致病性及对7种植物病原菌抑菌活性进行测定，结果表明，FLNXY5菌株菌丝生长最适温度为25 ℃，产孢量在28 ℃极显著高于其他处理。30与25 ℃处理下，24 h萌发率最高达91%，无显著差异。浓度为1.0×109孢子?mL-1的菌悬液处理小菜蛾2龄幼虫，5 d后的校正累计侵染率为89.2%。球孢白僵菌FLNXY5的拮抗机理研究中，菌株FLNXY5对水稻稻瘟病菌Pyricularia grisea菌丝抑制率最高达40.63%，对番茄灰霉病菌Botrytis cinerea菌丝抑制率最低为29.14%。本次研究得到的菌株发酵滤液对试验选取的7株植物病原菌菌丝没有抑制作用。

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备注/Memo

收稿日期：2019-07-15

基金项目：国家重点研发计划（2017YFD02011084）；国家科技重大专项和重点研发项目（课题）省级资金（GX18B017）。
第一作者简介： 杨帆(1982-)，女，博士，助理研究员，从事作物病虫害生物防治研究。E-mail：yf_echo2011@163.com。
通讯作者：李新民(1963-)，男，硕士，研究员，从事微生物源农药研究。E-mail：xinmin63@163.com。

更新日期/Last Update: 2020-05-04