[1]聂园军,李瑞珍,张春芬,等.草莓茎尖脱毒技术研究[J].黑龙江农业科学,2019,(03):29-32.[doi:10.11942/j.issn1002-2767.2019.03.0029]
　NIE Yuan-jun,LI Rui-zhen,ZHANG Chun-fen,et al.Study on Virus-free Technology of Strawberry Stem Tips[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(03):29-32.[doi:10.11942/j.issn1002-2767.2019.03.0029]
点击复制

草莓茎尖脱毒技术研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年03期 页码: 29-32 栏目: 出版日期: 2019-03-10
Title:
Study on Virus-free Technology of Strawberry Stem Tips
作者:
聂园军1; 李瑞珍1; 张春芬2; 邓舒2; 候丽媛3; 曹秋芬3
1.山西省农业科学院 农业资源与经济研究所，山西 太原 030006；2.山西省农业科学院 果树研究所，山西 太谷 030815；3.山西省农业科学院 生物技术研究中心，山西 太原 030031
Author(s):
NIE Yuan-jun1; LI Rui-zhen1; ZHANG Chun-fen2; DENG Shu2; HOU Li-yuan3; CAO Qiu-fen3
1.Institute of Agricultural Resources and Economics,Shanxi Academy of Agricultural Sciences,Taiyuan 030006,China; 2.Fruit Research Institute,Shanxi Academy of Agricultural Sciences,Taigu 030815,China; 3.Biotechnology Research Center,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China
关键词:
草莓; 脱毒技术; 匍匐茎再生
Keywords:
strawberry;  detoxification technology;  stolons regeneration
DOI:
10.11942/j.issn1002-2767.2019.03.0029
文献标志码:
A
摘要:
为建立操作简便、脱毒效率高的病毒脱除技术，促进草莓脱毒苗产业化发展，采用感染草莓斑驳病毒的草莓母株为材料，通过对草莓茎尖组织培养法、热培养处理后茎尖组织培养法、组培苗再生匍匐茎茎尖培养法等方式进行脱毒技术研究，比较几种脱毒技术手段的优缺点。结果表明：热处理后，草莓组培苗的脱毒率虽然比直接茎尖培养有所上升，但其成活率却下降。而组培苗再生匍匐茎茎尖培养法与直接茎尖培养法、热培养处理后茎尖培养法相比，在相同茎尖长度时，脱毒率和生存率更高，且茎尖长度为0.8~1.0 mm时，其存活率和成活率达到93.3%和96.4%。因此，组培苗再生匍匐茎茎尖培养法对草莓脱毒是比较有效的方法。
Abstract:
In order to establish a simple and efficient virus removal technology and promote the industrialization development of virus-free strawberry seedlings,the virus-free technology of strawberry mother strain infected with strawberry mottle virus was studied by tissue culture of strawberry stem tip,tissue culture of stem tip after heat culture and regeneration of stolon tip of tissue culture seedlings.Advantages and disadvantages were compared.The result showed that the detoxification rate of strawberry tissue culture seedlings increased,but the survival rate decreased after heat treatment.Compared with the direct stem tip culture method and the heat culture method after the stem tip culture,the virus-free rate and survival rate of the stem tip culture method with the same stem tip length were higher,and the survival rate and survival rate reached 93.3% and 96.4% when the stem tip length was 0.8-1.0 mm.Therefore,the regeneration of stolon tip culture with tissue culture was an effective method for strawberry detoxification.

参考文献/References:

［1］忻雅，吴根良，童建新，等.草莓工厂化育苗基质的筛选[J].浙江农业科学，2011（6）：1232-1235.

[2]李靖,王坷,楚新伟,等.草莓半促成栽培品种比较试验[J].河南农业科学,2004(8):61-63.
[3]张欣馨，王菲，李浪，等.中国草莓生产中面临的主要问题及发展对策[J].中国林副特产，2016（2）：92-96.
[4]何欢乐，阳静，蔡润，等.草莓茎尖培养脱毒效果研究[J].北方园艺，2005（5）：79-81.
[5]李志强,王晶,丁国亮,等.草莓热处理结合茎尖脱毒技术研究[J].北方园艺,2012（5）:125-127.
[6]陈怀勐，高丽，路河，等.红颜草莓茎尖组培脱毒技术研究[J].北京农业，2011（1）：18-20.
[7]高慧卿，樊兰瑛，王秀红，等.茎尖培养及热处理技术在百合脱毒中的应用研究[J].山西农业大学学报，2010，30（6）：528-532.

相似文献/References:

[1]梁文卫.草莓设施栽培研究进展[J].黑龙江农业科学,2014,(06):155.
　LIANG Wenwei.Research Progress of Strawberry for Facility Cultivation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(03):155.
[2]刘炳福,刘爱娜,张吉祥.99.8%硫磺粉熏蒸加喷咪酰胺对棚室草莓白粉病的影响[J].黑龙江农业科学,2012,(09):56.
　LIU Bing-fu,LIU Ai-na,ZHANG Ji-xiang.Effect of 99.8% Sulphur Fumigation Combined with Spraying Microphone Amide on Greenhouse Strawberry Powdery Mildew[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(03):56.
[3]兰 伟,万凌云,马宗新,等.三个草莓品种的组织培养研究[J].黑龙江农业科学,2012,(08):21.
　LAN Wei,WAN Ling-yun,MA Zong-xin,et al.Study on Tissue Culture of Three Varieties of Strawberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(03):21.
[4]刘建军,周金梅.草莓花药培养中诱导不定芽情况研究[J].黑龙江农业科学,2011,(11):13.
　LIU Jian-jun,ZHOU Jin-mei.Study on the Inducing Adventitious Buds inAnther Culture of Fragaria×ananassa in vitro[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(03):13.
[5]张玉红,吴业东,张 霞.寒地草莓设施栽培关键技术[J].黑龙江农业科学,2011,(11):52.
　ZHANG Yu-hong,WU Ye-dong,ZHANG Xia.Key Facilities Cultivation Techniques of Strawberry in Cold Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(03):52.
[6]赵海红.童子1号草莓茎尖分化最优培养基筛选研究[J].黑龙江农业科学,2010,(06):13.
　ZHAO Hai-hong.Screening of Optimum Medium on Tip Culture of Strawberry Tongzi No.1[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(03):13.
[7]王 超.西藏墨脱野生草莓属植物的调查和分类研究[J].黑龙江农业科学,2014,(01):88.
　WANG Chao.Investigation and Taxonomy of Wild Strawberry(Fragaria)Species in Motuo Country of Tibet[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(03):88.
[8]张辉明,姜永平,朱加平.不同生态型栽培基质配比对草莓生产的影响[J].黑龙江农业科学,2015,(01):69.[doi:10.11942/j.issn1002-2767.2015.01.0069]
　ZHANG Hui-ming,JIANG Yong-ping,ZHU Jia-ping.Effect of Different Proportion of Ecological Culture Media on Strawberry Production[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(03):69.[doi:10.11942/j.issn1002-2767.2015.01.0069]
[9]曾洪学.盐胁迫条件下草莓的生理生态表现及提高草莓耐盐性的途径[J].黑龙江农业科学,2015,(03):43.[doi:10.11942/j.issn1002-2767.2015.03.0043]
　ZENG Hong xue.Physiological and Ecological Performance of Strawberry Under Salt Stress and the Ways to Improve the Salt Tolerance of Strawberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(03):43.[doi:10.11942/j.issn1002-2767.2015.03.0043]
[10]赵海红.二次茎尖脱毒法对草莓茎尖培养及脱毒效果的影响[J].黑龙江农业科学,2015,(06):15.[doi:DOI:10.11942/j.issn1002-2767.2015.06.0015]
　ZHAO Hai-hong.Effect of Second Time Stem Apex Detoxification on Shoot Tip Cultures and Detoxification Efficiency of Strawberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(03):15.[doi:DOI:10.11942/j.issn1002-2767.2015.06.0015]

备注/Memo

收稿日期：2018-10-18

基金项目：山西省农业科学院特色农业技术攻关项目（YGG17117)。
第一作者简介：聂园军（1987-），男，硕士，助理研究员，从事设施园艺作物高产高效栽培技术研究。E-mail:nie379@126.com。

更新日期/Last Update: 2019-03-24