[1]李艳丽,邓百万,黄曼曼,等.鸡枞菌分离及生长条件研究[J].黑龙江农业科学,2017,(10):87-91.[doi:10.11942/j.issn1002-2767.2017.10.0087]
　LI Yan-li,DENG Bai-wan,HUANG Man-man,et al.Isolation and Growth Conditions of Termitomyces albuminosus[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(10):87-91.[doi:10.11942/j.issn1002-2767.2017.10.0087]
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鸡枞菌分离及生长条件研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年10期 页码: 87-91 栏目: 出版日期: 2017-10-10
Title:
Isolation and Growth Conditions of Termitomyces albuminosus
文章编号:
1002-2767（2017）10-0087-05
作者:
李艳丽1; 邓百万1; 2; 黄曼曼1; 解修超1; 2; 彭浩1; 2
1.陕西理工大学 生物科学与工程学院，陕西 汉中 723000；2.陕西省食药用菌工程技术研究中心，陕西 汉中723000
Author(s):
LI Yan-li1; DENG Bai-wan1; 2; HUANG Man-man1; XIE Xiu-chao1; 2; PENG Hao1; 2
1.School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723000；2.Shaanxi Engineering Research Center of Edible and Medicated Fungi,Hanzhong,Shaanxi 723001
关键词:
鸡枞菌; 分离; 鉴定; 生长条件
Keywords:
Termitomyces albuminosus; isolation; identification; growth conditions
分类号:
S646.1+9
DOI:
10.11942/j.issn1002-2767.2017.10.0087
文献标志码:
A
摘要:
为解决鸡枞菌分离较难的问题，对采自秦岭地区的一株野生鸡枞菌，采用组织分离法对标本菌株进行分离纯化，通过平皿观察法和ITS序列分析确定其菌属，以菌丝生长速率为指标、探索其最优生长碳源、氮源、KH2PO4浓度、MgSO4浓度、温度及酸碱度等培养条件。结果表明：经形态特征及ITS序列分析确定此菌株为鸡枞菌，该菌营养菌丝生长最适碳源是玉米粉；最适氮源是硝酸铵；鸡枞菌生长较快的KH2PO4浓度在0.10％~0.20％时，最适浓度是0.15％；MgSO4浓度在0.025％~0.075％时，最适浓度是0.050％；生长温度范围在25~27 ℃时，最适温度是26 ℃；pH范围5.0~6.0，最适pH是5.0。
Abstract:
To solve the difficult problem of separating Termitomyces albuminosus,the isolation,identification and growth characteristics of Qinling Mountains area from a wild Termitomyces albuminosuswere studied,tissue isolation method was used to isolate and purify the strains,its genus was determined by plate observation and ITS sequence analysis,the optimum growth carbon source,nitrogen source,KH2PO4 concentration,MgSO4 concentration,temperature and pH were investigated with the growth rate of mycelium as index.The results showed that according to the morphological characteristics and ITS sequence analysis to determine the strain of ?Termitomyces albuminosus,the optimum carbon source for the growth of mycelia was corn flour; the optimum nitrogen source was ammonium nitrate; the concentration of KH2PO4 was 0.10%~0.20%,the optimum concentration was 0.15%; the concentration of MgSO4 was 0.025%~0.075%,the optimum concentration was 0.050%; the growth temperature range was 25~27 ℃,the optimum temperature was 26 ℃; the growth pH range was 5.0~6.0,the optimum pH was 5.0.

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备注/Memo

收稿日期：2017-08-29

基金项目：2016年科技厅科技创新工程资助项目（2016HBGC-07）
第一作者简介：李艳丽(1990-)，女，陕西省韩城市人，在读硕士，从事微生物资源保育及开发利用研究。E-mail:1446727949@qq.com。
通讯作者：邓百万(1963-)，男，陕西省眉县人，博士，教授，从事微生物资源保育与开发利用研究。E-mail:2210309868@qq.com。

更新日期/Last Update: 2018-01-31