HAO Jia-chen.Comparison on Juglans mandshurica Pollen Viability Determination Method[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(01):67-68.[doi:10.11942/j.issn1002-2767.2017.01.0067]
核桃楸花粉活力测定方法比较
- Title:
- Comparison on Juglans mandshurica Pollen Viability Determination Method
- 文章编号:
- 1002-2767（2017）01-0067-02
- Keywords:
- Juglans mandshurica; pollen viability; staining; germination rate
- 分类号:
- S759.3
- 文献标志码:
- A
- 摘要:
- 为建立核桃楸高效快速的花粉活力测定方法，分别采用了TCC染色法、I2-KI染色法和固体培养基萌发法对核桃楸花粉活力进行了测定比较。结果表明：采用TCC染色法和I2-KI染色法染色后花粉的不易辨识，测定结果偏低，不适合作为核桃楸花粉活力的测定方法；固体培养基萌发法辨识度高，萌发率高，适合作为核桃楸花粉活力的测定方法。
- Abstract:
- In order to find out the quicker and easier methods to determination the pollen viability of Juglans mandshurica,the pollen viability were detected by methods of I2-KI,T TC and in vitro germination.According to the results methods，it was difficult to determine the pollen viability by the staining with TTC and I2-KI,and in vitro germination could be used to detect the pollen viability.
参考文献/References:
[1]黄桂龙,毛立仁,刘玉凤.核桃楸的经济价值及栽培技术[J].辽宁林业科技,2010(3):52-54.
[2]张超仪,耿兴敏.六种杜鹃花属植物花粉活力测定方法的比较研究[J].植物科学学报,2012,30(1):92-99．
[3]王燕哲,崔彦宏,张丽华,等.玉米花粉活力测定方法的比较研究[J].玉米科学,2010,18(3):173 -176．
[4]俞奔驰,李军,盘欢,等.木薯花粉活力测定及验证试验[J].广东农业科学,2012(13):25-27.
[5]汪妮,张志轩,董自梅.韭菜花粉生活力测定方法的筛选研究[J].长江蔬菜,2009(2):53-54.
[6]胡适宜.花粉生活力的测定[J].植物学通报,1993,70(2):60-62.
相似文献/References:
[1]明晓,吴璇,汉由之,等.黄毛草莓花粉活力及柱头可授性测定[J].黑龙江农业科学,2012,(05):80.
MING Xiao,WU Xuan,HAN You-zhi,et al.Pollen Viability and Stigma Receptivity of Fragaria nilgerrensis [J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(01):80.
[2]田英,王娅丽,王钰,等.文冠果传粉生物学特性研究[J].黑龙江农业科学,2013,(06):50.
TIANYing,WANGYa-li,WANGYu,et al.StudyonthePollinationBiologyCharacteristicofXanthocerassorbifoliaBunge[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):50.
[3]庄倩倩,陈少鹏,郑永春,等.栽培密度对长白山核桃楸苗期生长的影响[J].黑龙江农业科学,2013,(08):75.
ZHUANG Qian-qian,CHEN Shao-peng,ZHENG Yong-chun,et al.Effect of Planting Density on Seedling Growing of Juglans mandshurica from Changbai Mountains[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):75.
[4]付超,贾彩云,宋红霞.花龄对白菜花粉活力的影响[J].黑龙江农业科学,2013,(11):9.
FU Chao,JIA Cai-yun,SONG Hong-xia.Effects of Flowering Stage on Pollen Viability of Chinese Cabbage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):9.
[5]郑凯,丁久玲,梁慧敏.空气凤梨人工授粉技术研究[J].黑龙江农业科学,2018,(05):70.[doi:10.11942/j.issn1002-2767.2018.05.0070]
ZHENG Kai,DING Jiu-ling,LIANG Hui-min.Study on Artificial Pollination of Tillandsia stricta[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(01):70.[doi:10.11942/j.issn1002-2767.2018.05.0070]
[6]孟宪明,田梦飞,冯昌寅,等.HPLC法同时测定核桃楸枝皮中四种植物激素[J].黑龙江农业科学,2021,(04):104.[doi:10.11942/j.issn1002-2767.2021.04.0104]
[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(01):104.[doi:10.11942/j.issn1002-2767.2021.04.0104]
备注/Memo
收稿日期：2016-12-16
作者简介：郝家臣（1986-）,男，辽宁省大连市人，学士，助理工程师，从事经济林栽培研究。