KANG Zhexiu,WU Jingji,LANG Xianbo,et al.Establishment of the Regeneration System of Potato Variety Yanshu 4[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(12):11-13.[doi:10.11942/j.issn10022767.2016.12.0011]
马铃薯延薯4号再生体系建立
- Title:
- Establishment of the Regeneration System of Potato Variety Yanshu 4
- 文章编号:
- 10022767（2016）12001102
- Keywords:
- potato; Yanshu 4; stem segment; callus induction; plant regeneration
- 分类号:
- S532
- 文献标志码:
- A
- 摘要:
- 为了建立马铃薯高效、稳定离体再生体系，以延薯4号无菌试管苗茎段为材料，通过对茎段再生体系的筛选，优化了培养基内最佳激素配比浓度。结果表明：马铃薯延薯4号无菌试管苗茎段诱导愈伤组织最适培养基为：MS+6BA3.0 mg·L-1 +NAA1.0 mg·L-1。茎段丛生芽分化的最适培养基为：MS+6BA2.0 mg·L-1 +NAA0.20 mg·L-1 +G A3 1.0 mg·L-1。
- Abstract:
- To establish the highly efficient and stable in vitro regeneration system of potato,the stem regeneration system of the aseptic Yanshu 4 seedlings was screened and the hormone proportion of culture medium was optimized.The results showed that the optimum culture medium for the callus induction of explants from stem segments of Yanshu 4 was MS+6BA3.0 mg·L-1+NAA1.0 mg·L-1 and the best culture medium for differentiation of multiple buds inducted by stem segment callus was MS + 6BA2.0 mg·L-1+NAA0.20 mg·L-1+GA3 1.0 mg·L-1.
参考文献/References:
[1] 高志民 . 我国马铃薯主粮化战略启动 [N] ．人民政协报， 20150108 （ 07 ） . [2]李娟，程慧，张国裕.马铃薯叶片高效再生体系的建立[J].西北植物学报，2004（4）： 610614. [3]杨琼芬，卢丽丽，潘哲超，等.马铃薯叶片愈伤组织再生体系的建立[J].西南农业学报，2012（3）： 10011004. [4]鲍红春，李小雷，王建平，等.马铃薯品种陇薯5号茎段再生体系的建立[J].内蒙古农业科技，2014（2）： 3132. [5]李文霞，宁海龙，张永根，等.二倍体马铃薯花药培养再生体系的优化[J].中国蔬菜，2012（04X）：6874. [6]李晶.马铃薯再生体系的建立及遗传转化的研究[D]．哈尔滨：东北农业大学，2003. [7]柳容.马铃薯的再生及其再生植株遗传稳定性研究[D]．长沙：湖南农业大学，2007.
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备注/Memo
收稿日期：20161107