SHEN Yu,GAO Yan-ling,FAN Guo-quan,et al.Comparison of DAS-ELISA and RT-PCR Method for Detection of Potato Virus S[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(10):58-60.[doi:10.11942/j.issn1002-2767.2016.10.0058]
DAS-ELISA与RT-PCR法检测马铃薯S病毒的比较
- Title:
- Comparison of DAS-ELISA and RT-PCR Method for Detection of Potato Virus S
- 文章编号:
- 1002-2767（2016）10-0058-03
- 分类号:
- Q789；S532
- 文献标志码:
- A
- 摘要:
- 在马铃薯种薯生产中，马铃薯S病毒是田间发病率较高的病毒之一，同时也是最不易脱掉的病毒。为了更准确地筛查马铃薯病毒病，减少病毒病在田间的侵染，保证种薯质量，提高种薯产量，用DAS-ELISA和RT-PCR两种方法对马铃薯S病毒筛查的效果进行了比较。结果表明：两种方法间有较高的吻合度，但也存在一定的差异。DAS-ELISA相对RT-PCR而言成本更低效率更高，但灵敏度略低。同一条件下，对于同一样本，不同的试验方法会对试验结果有一定的影响。因此，在病毒检测工作中，应根据检测的实际要求，谨慎合理选取正确的方法，灵活利用RT-PCR进行补充检测，以保证检测及科研工作结果更加可靠。
- Abstract:
- Potato virus S (PVS) is one of the viruses which causes high rate of incidence in the seed potatoes production,and it is also the hardest one to free.Therefore,it appears frequently in the fields.In order to screen the potato virus disease more accurately,reduce the infection of virus in the field,ensure the quality of seed potato,increase the seed potato yield,the effect of virus detection by DAS-ELISA and RT-PCR methods were compared and studied.The results showed that two methods had a good agreement ,but there are some differences,too.Compared with RT-PCR,DAS-ELISA was lower costly,higher efficient,but the sensitivity was lower.Different methods in same situations have a certain effect on the experimental results.Therefore,in the virus detection work,correct method should be chosen based on the actual requirements of the detection,carefully and reasonably.RT-PCR method should be used to complement the detection,to ensure that the results of testing and scientific researchwas more reliable.
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备注/Memo
收稿日期：2016-08-06