[1]孙培元,王俊强,韩业辉,等.玉米RNA结合蛋白 GRMZM2G052926 基因的生物信息学分析[J].黑龙江农业科学,2016,(03):7-11.[doi:10.11942/j.issn1002-2767.2016.03.0007]
　SUN Pei-yuan,WANG Jun-qiang,HAN Ye-hui,et al.Bioinformatics Analysis of the RNA-binding Protein?GRMZM2G052926??Gene in Maize[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(03):7-11.[doi:10.11942/j.issn1002-2767.2016.03.0007]
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玉米RNA结合蛋白 GRMZM2G052926 基因的生物信息学分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2016年03 页码: 7-11 栏目: 出版日期: 2016-03-10
Title:
Bioinformatics Analysis of the RNA-binding Protein?GRMZM2G052926??Gene in Maize
文章编号:
1002-2767（2016）03-0007-05
作者:
孙培元1; 王俊强1; 韩业辉1; 于运凯1; 许 健1; 周 超1; 李 昕2
1.黑龙江省农业科学院 齐齐哈尔分院，黑龙江 齐齐哈尔 161041；2.黑龙江省农业科学院 五常水稻研究所，黑龙江 五常 150229
Author(s):
1.Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihar,Heilongjiang 161041; 2.Wuchang Rice Research Institute,Heilongjiang Academy of Agricultural Science,Wuchang,Heilongjiang 150229
关键词:
玉米; RNA结合蛋白;  GRMZM2G052926 基因; 生物信息学
Keywords:
maize;  RNA-binding protein;  ??GRMZM2G052926?gene;  bioinformatics analysis
分类号:
S513
DOI:
10.11942/j.issn1002-2767.2016.03.0007
文献标志码:
A
摘要:
为了研究玉米RNA结合蛋白在玉米生长发育中的调节机制，以一个玉米RNA结合蛋白 GRMZM2G052926 基因为材料，采用生物信息学的方法，对其理化性质、跨膜结构域、亚细胞定位、功能域、二级结构等方面进行了预测和分析。结果表明：基因包含一个1 335 bp开放阅读框，是一种具有亲水能力的不稳定酸性蛋白，相对分子质量38.301 1 kDa，包含两个RRM超亲家族（RRM superfamily），无信号肽序列，无跨膜结构域，亚细胞定位在细胞质上，二级结构由无规则卷曲、α螺旋、延伸链和β折叠构成；系统进化树分析表明，GRMZM2G052926蛋白与水稻Os05g0437300蛋白亲缘关系最近，同源性达到87%。
Abstract:
In order to study the regulatory mechanism of the maize RNA-binding protein in the maize growth and development，taking a RNA-binding protein ?GRMZM2G052926 ?gene as materials， its characters,including the physical and chemical properties,transmembrane domain,subcellular localization,functional domain plus the secondary structure were studied by bioinformatics tools.The results showed that this RNA-binding protein was an unstable hydrophilic acidic protein containing an open reading frame of 1 335 bp.The molecular weight of the protein containing two RRM super families was 38.301 1 kDa.It was no signal peptide,no transmembrane domain,located in the cytoplasm,and contained random coil,alpha-helix,extended strand and beta turn.The phylogenetic results showed that GRMZM2G052926 protein from Zea mays and Os05g0437300 protein from Oryza sativa were the nearest in genetic relationship,reaching to 87%.

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备注/Memo

收稿日期：2016-01-13

第一作者简介：孙培元（1991-），男，黑龙江省绥化市人，硕士，研究实习员，从事玉米分子遗传育种研究。E-mail：562337271@qq.com。

更新日期/Last Update: 2016-04-07