HE Yi hang,HUANG San-yin,DENG Kai-yuan,et al.Effect of Different Concentrations of Three Kinds of Hormone on ?Callus Induction of ?Cirsium setosum Stem[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(09):26-31.[doi:10.11942/j.issn1002-2767.2015.09.0026]
不同浓度三种激素对小蓟茎段愈伤组织诱导影响
- Title:
- Effect of Different Concentrations of Three Kinds of Hormone on ?Callus Induction of ?Cirsium setosum Stem
- 文章编号:
- 002-2767(2015)09-0026-06
- Keywords:
- Cirsium setosum; stem; callus; induction
- 分类号:
- Q94 331;S567.23+9
- 文献标志码:
- A
- 摘要:
- 为了进一步开发和利用小蓟,以小蓟茎段为外植体,采用单因素及正交试验方法,探讨2,4-D、6-BA以及NAA三种激素对小蓟愈伤组织诱导的影响。结果表明:单因素试验2,4-D最佳浓度范围为0.6~1.0 mg·L-1;6-BA最佳浓度范围为0.6~1.0 mg·L-1;NAA最佳浓度范围为2.4~2.6 mg·L-1。正交试验结果显示,在MS+2,4-D 1.0 mg·L-1+6-BA 1.0 mg·L-1+NAA 2.4 mg·L-1培养基中,小蓟茎段愈伤组织诱导效果最好。3种激素不同程度上促进小蓟愈伤组织的形成及生长。?
- Abstract:
- In order to further develop and use ?Cirsium setosum,the effect of 2,4-D,6-BA and NAA for Cirsium setosum ?callus induction were discussed by single factor and orthogonal test.The results showed that under single factor,2,4-D optimal concentration range from 0.8 to 1.0 mg·L-1,6-BA range from 0.8 to 1.0 mg·L-1?and NAA range from 2.4 to 2.6 mg·L-1.Orthogonal test results showed that the MS + 2,4-D 1.0 mg·L-1+6- BA 1.0 mg·L-1+NAA 2.4 mg·L-1?medium induced best callus.The three kinds of hormones all could promote Cirsium setosum ?stem callus formation and growth.?
参考文献/References:
[1]中国科学院植物志编辑委员会.中国植物志(第一分册)[M].北京:科学出版社,1991:569.?
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备注/Memo
收稿日期:2015-04-22?