LIU Chun-lai,LI Xin-min,WANG Shuang,et al.Preliminary Study on Isolating Antagonistic Bacteria from Soil and Antagonistic Activity Against Plant Pathogens[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(07):56-61.[doi:10.11942/j.issn1002-2767.2015.07.0056]
土壤拮抗细菌的分离与抗植物病原真菌活性初步研究
- Title:
- Preliminary Study on Isolating Antagonistic Bacteria from Soil and Antagonistic Activity Against Plant Pathogens
- 文章编号:
- 1002-2767（2015）07-0056-06
- Keywords:
-
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- 分类号:
- S432.23
- 文献标志码:
- A
- 摘要:
- 为了分离筛选出土壤中对植物病原真菌具有拮抗作用的细菌，以4种土传病害病原菌为诱导菌株，采用土壤颗粒撒布法，从不同生态区农田土壤样本中分离得到55株拮抗细菌菌株。测定了部分拮抗菌株及发酵滤液对植物病原菌的拮抗作用。结果表明：分离获得的细菌菌株对病原真菌均表现出一定程度的抑制作用，其中对菜豆根腐病菌(Fusarium solani)、小麦赤霉病菌(Fusarium graminearum)、番茄枯萎病菌(Fusarium oxysporum)菌丝抑制率达60%以上的拮抗菌株，分别有1、12和8株。9株拮抗菌发酵滤液对大豆菌核病菌（Sclerotinia sclerotiorum）、水稻稻瘟病菌（Pyricularia grisea）、玉米大斑病菌（Setosphaeria turcica）、小麦根腐病菌（Bipolaris sorokiniana）、水稻恶苗病菌（Fusarium moniliforme）和马铃薯早疫病菌（Alternaria solani）6种植物病原菌表现出较高的抑菌效果，其中YS7菌株发酵产物对玉米大斑病菌、小麦根腐病菌、水稻恶苗病菌均表现较强的抑菌活性，对玉米大斑病菌抑菌圈直径最大达到了56.2 mm。从抗菌谱上看，27株拮抗菌株代谢产物对4种以上的拮抗对象表现有抑菌活性，表明拮抗菌株代谢产物具有较为广谱的抑菌效果。
- Abstract:
- In order to screen antagonistic bacteria in soil for plant pathogens bio-control.55 antagonistic bacteria strains were isolated from soil samples in different ecological regions by the soil sprinkling method baited with 4 soil borne diseases.Part of antagonistic bacteria strains were evaluated for the antagonistic activity against plant pathogens.The result showed that more than 60% mycelial inhibition against ?Fusarium solani,Fusarium graminearum,Fusarium oxysporum??were 1,12 and 8 stains,respectively.Fermentation filtrate of 9 antagonistic bacteria strains showed higher inhibition effect for ?Sclerotinia sclerotiorum,Pyricularia grisea,Setosphaeria turcica,Bipolaris sorokiniana,Fusarium moniliforme and Alternaria solani,the fermentation products of YS7 strain showed strong antifungal activity to Setosphaeria turcica,Bipolaris sorokiniana,Fusarium moniliforme,the diameter of inhibition zone reached a maximum of 56.2 mm against Setosphaeria turcica.Metabolites of 27 antagonistic bacteria strains showed inhibition activity to more than 4 antagonistic objects,it showed that the fermentation products of antagonistic bacteria strains had broad-spectrum antifungal activity.
参考文献/References:
[1]Cook R J.Making greater use of introduced microorganisms for biological control of plant pathogens[J].Annual Review of Phytopathology,1993,33:53-80.
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备注/Memo
收稿日期：2015-02-10