BAO Yan-min,WANG Jia-min,MA Qi,et al.Growth and Metabolism Kinetic Research of MDCK Cells in Stationary Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(06):71-73,74.[doi:10.11942/j.issn1002-2767.2015.06.0071]
静置培养MDCK细胞的生长及代谢动力学研究
- Title:
- Growth and Metabolism Kinetic Research of MDCK Cells in Stationary Culture
- 文章编号:
- 1002-2767（2015）06-0071-03
- Keywords:
- MDCK cell; growth characteristics; metabolism kinetics
- 分类号:
- Q254
- 文献标志码:
- A
- 摘要:
- 为研究MDCK细胞的生长及代谢动力学特征，试验采用低密度静置培养，每天测定细胞生长密度和活力，并利用多参数生化分析仪测定培养液中Gluc、Lac、Gln和NH4+的含量，计算细胞指数生长期的比代谢速率。结果表明：MDCK细胞生长曲线呈“S”型，最大增殖密度为53.8×104 upf?mL-1，倍增时间为24.2 h；对数生长期Gluc和Gln的比代谢（消耗速率为－2.20 mg?(106cells?d)-1和－3.85 μmol?(106cells?d)-1，Lac和NH4+的比生成）速率为2.25 mg?(106cells?d)-1和2.22 μmol?(106cells?d)-1。
- Abstract:
- n order to research growth and metabolism dynamics characteristics of MDCK cells,low cell density cultured method was used to determine cell density and viability every day.The content of Gluc,Lac,Gln and NH4+ in medium were determined by multiparameter biochemical analyzer,and the specific metabolic rate were calculated.The results showed that the growth curve of MDCK cells was “S” type,the largest density was 53.8×104 upf?mL-1 and the doubling time was 24.2 h.Specific consumption rate of Gluc and Gln were －2.20 mg?(106cells?d)-1 and －3.85 μmol?(106cells?d)-1 in logarithmic phase,and specific generation rate of Lac and NH4+ were 2.25 mg?(106cells?d)-1 and 2.22 μmol?(106cells?d)-1.
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备注/Memo
收稿日期：2015-02-04
基金项目：国家民委领军人才计划资助项目；国家自然科学基金资助项目（31160033）；甘肃省科技计划资助项目（1308RJZA142）；西北民族大学中央高校基本科研业务费专项资金资助项目（31920150029，31920130066）
第一作者简介：暴艳敏（1984-），女，辽宁省朝阳市人，在读硕士，从事动物疫苗开发和质量控制研究。E-mail：286330873@qq.com。
通讯作者：乔自林（1976-），男，甘肃省永靖县人，学士，高级实验师，从事动物细胞生物反应器高密度培养工艺研究。E-mail：670267497@qq.com。