[1]张立微,张景涛,李小梅.芫荽软腐病病原菌分离与鉴定[J].黑龙江农业科学,2015,(06):48-51.[doi:DOI:10.11942/j.issn1002-2767.2015.06.0048]
　ZHANG Li-wei,ZHANG Jing-tao,LI Xiao-mei.Separation and Identification of Erwinia caratovora ssp.caratovora Pathogen of Coriandrum sativum L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(06):48-51.[doi:DOI:10.11942/j.issn1002-2767.2015.06.0048]
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芫荽软腐病病原菌分离与鉴定

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2015年06 页码: 48-51 栏目: 出版日期: 2015-06-10
Title:
Separation and Identification of Erwinia caratovora ssp.caratovora Pathogen of Coriandrum sativum L.
文章编号:
1002-2767（2015）06-0048-04
作者:
张立微; 张景涛; 李小梅
哈尔滨市农业科学院,黑龙江 哈尔滨 150029
Author(s):
ZHANG Li-wei; ZHANG Jing-tao; LI Xiao-mei
Harbin Academy of Agricultural Sciences，Harbin,Heilongjiang 150029
关键词:
芫荽; 软腐病; 生理生化
Keywords:
Coriandrum sativum?L.;  Erwinia caratovora subsp.caratovora;  physiology and biochemistry
分类号:
S436.36
DOI:
DOI:10.11942/j.issn1002-2767.2015.06.0048
文献标志码:
A
摘要:
为有效防治芫荽软腐病，提高其产质量，通过对引起芫荽腐烂而绝产的病害的病原菌进行分离、纯化,对19个菌株进行17项生理生化指标测定。结果表明：根据伯杰氏细菌鉴定手册（第八版）以Ecc为对照进行鉴定，确定E1（Ames24923）、E3（Ames24927）、E4（CORI107）、E7（CORI139）、E8（CORI140）、E10（CORI147）、E12（CORI289）、E13（CORI318）、E18（PI664510）为芫荽胡萝卜欧文氏杆菌属胡萝卜软腐欧文氏杆菌胡萝卜软腐病亚种(Erwinia carotovora ssp.carotovora,Ecc) 细菌，而其余供试菌株通过测定可确定为细菌，但属何种还需进一步鉴定。
Abstract:
In order to control the Erwinia caratovora?ssp.caratovora?of Coriandrum sativum?L.,and improve the quality,through the isolating and purificating of the pathogen which causes coriander decay,19 strains were isolated and purified.The results showed that according to Bergey’s Manual of Systematic Bacteriology (eighth edition) and 17 tests of physiology and biochemistry,9 strainss were Erwinia carotovora?ssp.carotovora,including E1（Ames24923）,E3（Ames24927）,E4（CORI107）,E7（CORI139）,E8（CORI140）,E10（CORI147）,E12（CORI289）,E13（CORI318）,E18（PI664510）.Other strains should to be identified by other tools.

参考文献/References:

[1］孙广宇.植物病理学实验技术[M].北京:中国农业出版社,2002.

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[8]De Boer S H,Verdonck L,Vruggink H.,et al.Serological and biochemical variation among potato strains of Erwinia carotovora subsp.atroseptica and their taxonomic relationship to other Erwinia carotovora strains[J].J.Appl.Bacteriol,1987(63):487-495.

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　LI Xiao-mei,LUAN Jie,ZHANG Jing-tao,et al.Optimization of RAPD Reaction Conditions for?Coriander sativum [J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(06):9.
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备注/Memo

收稿日期：2014-10-08

第一作者简介：张立微（1982-）,女,黑龙江省绥化市人,硕士，农艺师，从事蔬菜遗传育种研究。E-mail：307832795@qq.com。
通讯作者：张景涛（1963-），男，硕士，高级农艺师，从事蔬菜遗传育种研究。E-mail：702444364@qq.com。

更新日期/Last Update: 2015-07-27