[1]罗茜,张海玲,徐香玲,等.Na+/H+逆向转运蛋白基因 PsnhaA 的克隆及在大豆中的功能验证[J].黑龙江农业科学,2015,(05):6-12.[doi:10.11942/j.issn1002-2767.2015.05.0006]
　LUO Qian,ZHANG Hai-ling,XU Xiang-ling,et al.Cloning of Na+/H+Antiporter Gene PsnhaA and Analysis on Function in Soybean[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(05):6-12.[doi:10.11942/j.issn1002-2767.2015.05.0006]
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Na+/H+逆向转运蛋白基因 PsnhaA 的克隆及在大豆中的功能验证

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2015年05 页码: 6-12 栏目: 出版日期: 2015-05-11
Title:
Cloning of Na+/H+Antiporter Gene PsnhaA and Analysis on Function in Soybean
文章编号:
1002-2767（2015）05-0006-07
作者:
罗茜1; 张海玲2; 徐香玲1; 姚琳1; 王全伟1
1.哈尔滨师范大学 生命科学与技术学院/黑龙江省分子细胞遗传与遗传育种重点实验室，黑龙江 哈尔滨150025；2.黑龙江省农业科学院，黑龙江 哈尔滨 150086
Author(s):
LUO Qian1; ZHANG Hai-ling2; XU Xiang-ling1; YAO Lin1; WANG Quan-wei1
1.Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province,College of Life Science and Technology,Harbin Normal University,Harbin,Heilongjiang 150025；2.Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086
关键词:
Na+/H+逆向转运蛋白; 克隆; 大豆; 遗传转化
Keywords:
Na+/H+antiporter gene; cloning; soybean; genetic transformation
分类号:
S567
DOI:
10.11942/j.issn1002-2767.2015.05.0006
文献标志码:
A
摘要:
高盐对作物造成渗透胁迫和离子毒害，Na+/H+逆向转运蛋白是生物耐盐的关键因子，能够维持高盐胁迫下生物体的正常生长代谢。为促进作物的遗传转化，选育耐盐作物品种，从施氏假单胞菌（Pseudomonas stutzeri）中克隆质膜Na+/H+逆向转运蛋白基因PsnhaA，并构建到植物表达载体pBI121上，通过发根农杆菌介导的整株生长点注射法转化大豆。结果表明：PsnhaA基因已整合到转化大豆基因组中，并在转录水平获得表达。耐盐相关生理指标检测结果显示，盐胁迫后转基因植株的质膜相对电导率显著低于对照植株，叶绿素含量和脯氨酸含量显著高于对照植株。PsnhaA基因显著提高了大豆的耐盐水平，为农作物的耐盐性改良提供了优良的候选基因。
Abstract:
In order to promote crop genetic transformation,breed salt-tolerant crop varieties,the osmotic stress and ion toxicity caused by high salt is one of the major abiotic stress factors that affect the crop growth and development.Na+/H+antiporter is the key factor in the salt stress tolerance in organism.It can maintain normal growth and metabolism of organism under high salt stress.A plasma membrane Na+/H+?antiporter gene nhaA was cloned from Pseudomonas stutzeri,and the plant expression vector pBI121 was constructed.PsnhaA gene was transformed into soybean growing point via Agrobacterium mediated transformation.The results showed that PsnhaA was integrated into the soybean genome,and could transcribed.Salt resistance analysis showed that the relative electronic conductivity of the transformed plants plasma membrane was significantly lower than that of the control under salt stress.While the content of chlorophyll and proline in the transformed plants were significantly higher than that in the controls.The expression of PsnhaA increased the salt stress tolerance of the transgenic soybean and provided excellent candidate genes for improving salt tolerance of crops

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备注/Memo

2014-12-25

黑龙江省自然科学基金重点资助项目（ZD201003）；哈尔滨市科技创新人才研究专项资金资助项目（RC2013QN002103）；黑龙江省教育厅科学技术研究资助项目（12511154）；哈尔滨师范大学博士科研启动基金资助项目（KGB200903）
第一作者简介：罗茜（1992-），女，黑龙江省龙江县人，学士，从事基因工程方面的相关研究。E-mail:luoqian1992@126.com。
通讯作者：王全伟（1978-），女，博士，副教授，从事植物分子遗传学与基因工程研究。E-mail:wqw125@126.com。

更新日期/Last Update: 2015-05-20