DENG Zheng-dong,CHENG Ai-fang,LI Xiu-li.Study on the Extraction and Agglutination of Lectin from Melastoma dodecandrum Lour[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(02):56-58.[doi:10.11942/j.issn1002-2767.2015.02.0056]
地稔凝集素的提取及凝血活性的研究
- Title:
- Study on the Extraction and Agglutination of Lectin from Melastoma dodecandrum Lour
- 文章编号:
- 1002-2767(2015)02-0056-03
- 分类号:
- Q946.1
- 文献标志码:
- A
- 摘要:
- 为研究和利用地稔的促凝血活性物质,以地稔为试材,提取地稔凝集素,研究pH、温度和糖等理化因素对凝血活性的影响。结果表明:经硫酸铵沉淀及透析提取的地稔凝集素具有红细胞凝集活性;碱性条件下,抑制作用明显,而酸性条件有较强促进作用;温度对凝集素凝血活性影响很小;半乳糖有明显促进作用。
- Abstract:
- In order to study and utilize the procoagulant activity substances from Melastoma dodecandrum Lour,taking Melastoma dodecandrum Lour as material,extraction of lectin and the effect of physical and chemical agents on agglutinating activity were studied,such as pH,temperature and sugar.The results showed that the lectin extracted by ammonium sulfate precipitation and dialysis had agglutinating activity.Alkaline conditions had significantly inhibited the agglutinating activity.While acidic conditions had a strong role in promoting.The effect of temperature on agglutinating activity was little.Galactose had an obvious effect on promoting.
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备注/Memo
收稿日期:2014-10-26
基金项目:武汉生物工程学院资助项目(2010J07)
第一作者简介:邓政东(1973-),男,广东省蕉岭县人,硕士,讲师,从事细胞生物学教学和科研工作。E-mail:dzddoron@sina.com。