[1]石玉涛,林小娥,郑淑琳,等.不同树龄武夷水仙茶多糖抗氧化活性研究[J].黑龙江农业科学,2014,(11):116-120.
　SHI Yu-tao,LIN Xiao-e,ZHENG Shu-lin,et al.Study on Antioxidant Activity of Tea Polysaccharides of C.sinensis cv.Fujian shuixian with Different Ages[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(11):116-120.
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不同树龄武夷水仙茶多糖抗氧化活性研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年11 页码: 116-120 栏目: 出版日期: 2014-11-10
Title:
Study on Antioxidant Activity of Tea Polysaccharides of C.sinensis cv.Fujian shuixian with Different Ages
文章编号:
1002-2767(2014)11-0116-05
作者:
石玉涛; 林小娥; 郑淑琳; 李远华
武夷学院 茶与食品学院/福建省武夷茶资源创新利用重点实验室/福建省高校茶叶工程研究中心/中国乌龙茶产业协同创新中心（培育），福建 武夷山 354300
Author(s):
SHI Yu-tao; LIN Xiao-e; ZHENG Shu-lin; LI Yuan-hua 
College of Tea and Food Science,Wuyi University/Fujian Provincial Key Laboratory of Wuyi Tea Resources Innovation and Utilization/Tea Engineering Research Center of Fujian Higher Education/Collaborative Innovation Center of Chinese Oolong Tea Industry(Breeding),Wuyishan,Fujian 354300
关键词:
武夷水仙; 树龄; 生化成分; 茶多糖; 抗氧化活性
Keywords:
C.sinensis cv.Fujian shuixian; tea plant age; chemical components; tea polysaccharides; antioxidant activity
分类号:
S571.1
文献标志码:
A
摘要:
为探明不同树龄武夷水仙茶叶中主要生化成分及其茶多糖的抗氧化活性的差异，以树龄为6、30、60 a的武夷水仙茶叶为试材，测定了主要生化成分含量，通过水提醇沉淀法提取茶多糖，分析了茶多糖的基本组分，并研究了3种茶多糖清除DPPH自由基和超氧阴离子自由基的活性。结果表明：3种水仙茶叶中茶多酚、游离氨基酸和黄酮类含量随着树龄的增长逐渐降低，可溶性糖含量随树龄的增长而升高；3种武夷水仙茶多糖的得率及其组分中的中性糖、蛋白质、糖醛酸、茶多酚的含量存在明显差异，茶多糖的得率随树龄的增长而提高，得率较高的老丛水仙茶多糖组分中的中性糖、蛋白质的含量也较高；3种茶多糖清除DPPH自由基的能力较为接近，其IC50分别为：0.051、0.055、0.060 mg?mL-1；3种茶多糖清除超氧阴离子自由基的能力存在明显差异，其IC50分别为：1.131、1.121、0.431 mg?mL-1，树龄为60 a的老丛水仙茶多糖清除超氧阴离子自由基活性明显高于树龄为6和30 a的水仙茶多糖，具有较强的抗氧化活性。
Abstract:
In order to explore the difference of chemical components and the activity of tea polysaccharides of C.sinensis cv.Fujian shuixian with different ages,taking three kinds of C.sinensis cv.Fujian shuixian as materials which tea plant ages were 6,30 and 60 years,the contents of flavonoids,free amino acids,soluble sugar,tea polyphenols and other major biochemical components were measured,as well as tea polysaccharides(TPS),contents of protein,neutral sugar,uronic acid and tea polyphenol.The scavenging activity of DPPH and O2?  by TPS were compared.The results showed that the contents of flavonoids,free amino acids and tea polyphenols decreased with the increasing of tea tree age,while the content of soluble sugar increased.There was a very significant difference in the contents of uronic acid,neutral sugar,protein and tea polyphenol of TPS,and the yield rate of TPS,the content of neutral sugar and protein in TPS increased with the increasing of tea tree age.The scavenging ability of DPPH of TPS with different tea tree ages were close to each other,IC50 were 0.051,0.055 and 0.060 mg?mL-1 respectively,the scavenging ability of O2? of TPS with different tea tree ages were significantly different, IC50 were 1.131,1.12 and 0.431 mg?mL-1 respectively,it illustrated that TPS of C.sinensis cv.Fujianshuixian for 60 years had better radical scavenger than the other two which possessed a strong antioxidant activity. 〖

参考文献/References:

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备注/Memo

收稿日期：2014-08-11基金项目：福建省教育厅A类科技资助项目[JA11260]；武夷学院青年教师专项科研基金资助项目[xq201014]；武夷学院大学生创新性试验计划资助项目[xj2011014]第一作者简介：石玉涛(1984-)，男，陕西省榆林市人，硕士，助教，从事茶叶生物化学与资源综合利用研究。E-mail:shiyt2010@163.com。通讯作者：李远华（1963-），男，福建省仙游县人，博士，教授，从事茶学及生物技术研究。E-mail:yhli@mail.wuyiu.edu.cn。

更新日期/Last Update: 2014-12-13