CHENG Wei-shun,ZENG Hong-xia,DU Nian-hua,et al.Effect of Droughtsalt Stress on Soluble Protein and Soluble Sugar of Watermelon Seedlings[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(11):91-93.
干旱-盐胁迫对西瓜幼苗可溶性蛋白质及可溶性糖的影响
- Title:
- Effect of Droughtsalt Stress on Soluble Protein and Soluble Sugar of Watermelon Seedlings
- 文章编号:
- 1002-2767(2014)11-0091-03
- 分类号:
- S667.7
- 文献标志码:
- A
- 摘要:
- 为探讨西瓜抗逆性及其丰产人工栽培技术,通过PEG 6000和0.1 mol?L-1Nacl处理分别模拟干旱和盐胁迫,并测定其可溶性蛋白质和可溶性糖的含量变化来对西瓜幼苗在干旱、盐逆境交叉适应的反应机制进行研究。结果表明:西瓜在经过单一盐胁迫后,其可溶性蛋白质含量明显下降,可溶性糖的含量明显增加;并且在干旱-盐交叉逆境中可溶性蛋白质含量下降幅度有所减小,可溶性糖的含量增加幅度有所提高。因此,可以初步断定,干旱预处理可以提高西瓜幼苗的耐盐性。
- Abstract:
- In order to study the stress resistance and high yield cultivation techniques of atermelon,drought salt stress was simulated by the treatments of PEG 6000 and 0.1 mol?L-1NaCl,the change of the soluble protein and sugar were measured to study the reaction mechanism of watermelon seedlings in cros sadaptation.The results showed that the soluble protein content of atermelon seedlings decreased and the soluble sugar content increased significantly in a single salt ess,and the two indicators increased in the cross-adaptation of drought salt stress.Therefore,it was initially concluded that drought pretreatment could improve the salt tolerance of watermelon seedlings.
参考文献/References:
[1]张志忠,吴菁华,吕柳新.根癌农杆菌介导的西瓜遗传转化研究[J].果树学报,2005,22(2):134137. [2]山仑,陈培元.旱地农业生理生态基础[M].北京:科学出版社,1998:23-24.
[3]Parida A K,Das A B.Salt tolerance and salinity effects on plants[J].Ecotoxicology and Environ mental Safe,2005,60:324-349.
[4]李彦,张英鹏,孙明,等.盐胁迫对植物的影响及植物耐盐机理研究进展[J].植物生理科学,2008,24(1):258265.
[5]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding[J].Anallytical Biochemistry,1976,72:248-54. [6]Yemm E W,Willis A J.The estimation of carbohydrates in plant extracts by anthrone[J].Biochemistry Journal,1954,57:508-514.
[7]孙小芳,郑青松,刘友良.NaCl胁迫对棉花种子萌发和幼苗生长的伤害[J].植物资源与环境学报,2000,9(3):2225.
[8]Wang Y N,Zhang W S,Li K X,et al.Saltinduced plasticity of root hair development is caused by ion disequilibrium in Arabidopsis thaliana[J].Plant Research,2008,121:87-96.
[9]王仁雷,华春,罗庆云,等.盐胁迫对植物的影响及植物盐适应性研究进展[J].生态学报,2007,20(12):5447-5454.
[10]谈建康,安树青.钠盐胁迫对小麦叶片核酸损伤和多胺积累的影响[J].农业环境科学学报,2004,23(3):428-431.
[11]张敏,蔡瑞国,李慧芝,等.盐胁迫环境下不同抗性玉米品种幼苗长势和内源激素的变化[J].生态学报,2008,28(1):310-320.
[12]林莺,李伟,范海,等.海滨锦葵光合作用对盐胁迫的响应[J].山东师范大学学报,2006,21(2):118-120.
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备注/Memo
收稿日期:2014-06-03基金项目:武汉市农业科学院2012年科技资助项目(Y120-1215
第一作者简介:程维舜(1984-),男,湖北省武汉市人,助理农艺师,硕士,从事植物与病原物互作分子生物学研究。E-mail:heart113@sina.com。
通讯作者:孙玉宏(1968-),女,湖北省武汉市人,硕士,正高级职称农艺师,从事西甜瓜栽培与育种研究。