REN Peng-bin,WANG A-jiao,LIU Jia-chen,et al.Effect of TDZ and 6-BA on Adventitious Bud Differentiation of Amorphophallus[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(08):23-27.
TDZ与6-BA对魔芋不定芽诱导的效应研究
- Title:
- Effect of TDZ and 6-BA on Adventitious Bud Differentiation of Amorphophallus
- 文章编号:
- 1002-2767（2014）08-0023-05
- Keywords:
- Amorphophallus; TDZ; 6-BA; adventitious buds
- 分类号:
- S632.3
- 文献标志码:
- A
- 摘要:
- 为了建立魔芋离体培养高效再生体系，研究了TDZ与6-BA对魔芋不定芽分化的效应。结果表明：（1）单独使用TDZ和6-BA或6-BA与TDZ、6-BA与NAA组合均能诱导魔芋愈伤组织分化形成不定芽，且TDZ与NAA组合有利于魔芋不定芽的分化，当培养基中激素配比为TDZ 1.0 mg.L-1+NAA 0.1 mg.L-1时，不定芽分化率可高达96.15%；（2）TDZ或TDZ与NAA组合对魔芋不定芽数量的增加非常有效，增殖系数显著高于单独使用6-BA或6-BA与NAA组合，培养基中TDZ为1.0 mg.L-1时增殖系数最高，可达15.78。（3）相比于激素6-BA，TDZ更易于导致不定芽的白化现象，最高白化率可达28.37%，而培养基中NAA的适当添加，可在很大程度上降低魔芋不定芽的白化。TDZ 1.0 mg.L-1与NAA 0.1 mg.L-1配比，不定芽白化率降至7.56%，6-BA 2.0 mg.L-1与NAA 0.1 mg.L-1配比不定芽白化率为0。（4）TDZ对魔芋不定芽的伸长生长表现出较强的抑制作用，而6-BA对不定芽的伸长生长则表现出较好的促进作用，可见在继代培养中使用6-BA更有利于魔芋不定芽的伸长生长。
- Abstract:
- In order to establish high-efficiency in vitro culture regeneration system,the effect of TDZ and 6-BA on adventitious bud differentiation of Amorphophallu was studied.The results showed that(1)Using TDZ or 6-BA singly and their combination with NAA could induce the differentiation of adventitious buds and the combination of TDZ and NAA was better,the differentiation rate had reached 96.15% when hormone concentration was TDZ 1.0 mg.L-1+NAA 0.1 mg.L-1.(2)Multiplication coefficient of TDZ or the combination with NAA attainted 15.78 which was higher than 6-BA and combination with NAA,there was 1.0 mg.L-1 TDZ,which demonstrated that TDZ had great effect on multiplication of adventitious buds.(3)Compared to 6-BA,TDZ was apt to bring whitening,and the highest whitening rate was 28.37%,however,NAA could largely decrease whitening.Whitening rate of NAA 0.1 mg.L-1 with TDZ 1.0 mg.L-1 and NAA 0.1 mg.L-1 with 6-BA 2.0 mg.L-1 fell to 7.56% and 0 respectively.(4)Differing from 6-BA,TDZ showed stronger suppression to growth of adventitious bud,so 6-BA was relatively appropriate in secondary culture of adventitious buds.
参考文献/References:
[1]刘佩瑛.魔芋学[M].中国.北京:农业出版社,2004:1-30.
[2]何亚娟,李久香.魔芋对艾氏腹水瘤(EAC)抑制作用的研究[J].肿瘤防治研究,2000(27):197-198.
[3]Sood N,Baker W L,Coleman C I.Effect of glucomannan on plasma lipid and glucose concentrations,body weight,and blood pressure:systematic review and meta-analysis[J].Am J Clin N,2008，88:1167-1175.
[4]Vuksan V,Sievenpiper J L,Zheng X.et al.Konjac-mannan and American Ginsing:Emerging alternative therapies for type 2 diabetes mellitus[J].J.Am.Coll.Nutr.,2001，20（5S）:370S-380S.
[5]Tamura M,Tsushida T，Shinohara K.Konjac glucomannan consumption may enhance equol production in mice[J].Food Sci.Technol.Res.,2005(11):376-379.
[6]徐炜,王婷婷,刘列平,等.陕西岚皋县魔芋软腐病原菌的分离与鉴定[J].西北农林科技大学学报:自然科学版,2011(11):97-102.
[7]杨英,何峰,金文闻,等.魔芋种植业发展存在的问题与对策[J].湖北农业科学,2008(9):1095-1098.
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备注/Memo
收稿日期：2014-04-01
基金项目：陕西省教育厅自然科学专项计划资助项目（2013JK0741）
第一作者简介：任鹏斌（1992-），男，陕西省铜川市人，学士，从事植物学研究。E-mail:15769162769@163.com。