CHEN Wen-wu.Study on Inducing Callus of Vitro Leaves of Pyracantha fortuneana[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):21-22.
火棘离体叶片愈伤组织的诱导研究
- Title:
- Study on Inducing Callus of Vitro Leaves of Pyracantha fortuneana
- 文章编号:
- 1002-2767（2014）07-0021-02
- Keywords:
- Pyracantha fortuneana; vitro leaves; callus; induction
- 分类号:
- S686
- 文献标志码:
- A
- 摘要:
- 为了获得优质的愈伤组织，以火棘的离体叶片为外植体，MS为基本培养基，研究2,4-D和6-BA以不同浓度组合，叶片的暗处理以及不同部位的叶片对愈伤组织诱导的影响。结果表明:培养基MS+2.0 mg·L-12,4-D+2.5 mg·L-16-BA对愈伤组织的诱导最好；暗处理10 d，然后转入光照下培养可提高叶片愈伤组织的诱导效果；较上部叶片愈伤组织诱导率可达96.7%。
- Abstract:
-
In order to obtain callus with high quality，taking the vitro leaves of Pyracantha fortuneana as explant and taking MS as basic medium,the effect of different concentrations of 2,4-D and 6-BA, as well as illumination time and different parts of leaves on callus induction of were studied.The results showed that the medium of MS+2.0 mg·L-1 2,4-D+2.5 mg·L-1 6-BA was the best for callus induction;dark treatment for 10 days，and then light cultivating could improve the effect of callus induction,callus induction rate of the upper leaves could amount to 96.7%.
参考文献/References:
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[2]陈文武,李清秀.火棘的茎尖培养与快速繁殖\[J\].北方园艺，2007（9）：202-203.
[3]崔美,焦其庆.苹果叶片愈伤组织的诱导培养\[J\].山东农业科学，2012(3):17-20.
[4]宁强,刘晓光.冬枣叶片愈伤组织诱导研究\[J\].河北林果研究，2008（4）：348-352.
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备注/Memo
收稿日期：2014-03-14
作者简介：陈文武（1975-），男，山东省陵县人，学士，讲师，从事生物技术和植物保护研究。E-mail：75413976@sina.com。