[1]沈 洁,袁堂茹,赵兴兰,等.蜈蚣兰初代培养中内生真菌的抑制作用[J].黑龙江农业科学,2014,(02):16-17.
SHEN Jie,YUAN Tang-ru,ZHAO Xing-lan,et al.Inhibition Effect of Endophytic Fungi During Primary Culture of Cleisostoma scolopendrifolium Garay[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):16-17.
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SHEN Jie,YUAN Tang-ru,ZHAO Xing-lan,et al.Inhibition Effect of Endophytic Fungi During Primary Culture of Cleisostoma scolopendrifolium Garay[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(02):16-17.
蜈蚣兰初代培养中内生真菌的抑制作用
《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S]
卷:
期数:
2014年02
页码:
16-17
栏目:
出版日期:
2014-02-10
- Title:
- Inhibition Effect of Endophytic Fungi During Primary Culture of Cleisostoma scolopendrifolium Garay
- Keywords:
- Cleisostoma scolopendrifolium Garay; preservative; potassium sorbate; sodium benzoate; sodium hypochlorite solution; endophytic fungi; inhibition
- 分类号:
- S682.31
- 文献标志码:
- A
- 摘要:
- 为了建立蜈蚣兰无菌繁殖体系,以0.2%的山梨酸钾和0.2%的苯甲酸钠为供试药剂,研究了两种不同种类的食品防腐剂在蜈蚣兰初代培养中对内生真菌的抑制作用。结果表明:两种食品防腐剂均对蜈蚣兰内生真菌的生长有抑制作用,且山梨酸钾的抑菌作用更为显著。同时,两种防腐剂的加入对外植体侧芽萌发均有一定程度的抑制,且苯甲酸钠的抑制作用较为突出。另外,0.8%的次氯酸钠溶液对蜈蚣兰内生真菌的生长也有一定的抑制作用。
- Abstract:
- In order to establish the sterile propagation system of Cleisostoma scolopendrifolium Garay,the inhibition on endophytic fungi during primary culture of Cleisostoma scolopendrifolium Garay were studied by 0.2% potassium sorbate and 0.2% sodium benzoate in medium.The results showed that both two kinds of preservatives had inhibition effect for the growth of fungi and the inhibition effect of 0.2% potassium sorbate was more significant.The addition of two kinds of preservatives had a certain inhibition for the germination of external lateral bud,and the inhibition of sodium benzoate was more prominent.In addition,0.8% sodium hypochlorite solution could inhibit the growth of endophytic fungi
参考文献/References:
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备注/Memo
收稿日期:2013-09-11 基金项目:2012年江苏省高等学校大学生实践创新训练计划资助项目(2012JSSPITP4290) 第一作者简介:沈洁(1969-),女,江苏省靖江市人,硕士,教授,从事植物细胞结构与分子生物学的教学与研究。E-mail:shenjie1969@126.com。
更新日期/Last Update:
2014-12-04