SONG Yan-dong,LIU Qing,ZHAO Heng-shou.Research on Acute Toxicity of Caulis spatholobi and Radix glycyrrhizae to Goldfish and Loach[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(10):65-68.
鸡血藤和甘草制剂对金鱼及泥鳅急性毒性的研究
- Title:
- Research on Acute Toxicity of Caulis spatholobi and Radix glycyrrhizae to Goldfish and Loach
- 文章编号:
- 1002-2767(2014)10-0065-04
- Keywords:
- millettial(Caulis spatholobi); liquorice(Radix glycyrrhizae); goldfish; loach; acute toxicity; LC50
- 分类号:
- S965.1;R285
- 文献标志码:
- A
- 摘要:
- 为了探究不同浓度鸡血藤(Caulis spatholobi)和甘草(Radix glycyrrhizae)制剂对不同鱼类的急性毒性,参照预试验结果将鸡血藤和甘草制剂分别添加至养殖水中,5个浓度梯度分组放养金鱼和泥鳅,每隔12 h观察死亡数并计数,96 h结束试验,分析鸡血藤和甘草对金鱼和泥鳅半致死浓度和安全浓度。结果表明:两种药物对金鱼的急性毒性为甘草>鸡血藤,对泥鳅的毒性为甘草<鸡血藤;两种鱼类对鸡血藤和甘草的耐受性为金鱼>泥鳅。鸡血藤对金鱼和泥鳅的半致死浓度和安全浓度分别为2.539 0、1.655 0 g.L-1和0.253 9、0.165 5 g.L-1;甘草对金鱼和泥鳅的半致死浓度和安全浓度分别为2.201 0、1.842 0 g.L-1和0.220 1、0.184 2 g.L-1。
- Abstract:
- Aiming at detecting the acute toxicity of different concentrations of millettia(Caulis spatholobi)and liquorice(Radix glycyrrhizae)preparations to goldfish and loach. Under the premise of the pre-test,two kinds of fish were divided into five groups with different concentration of millettia and liquorice.The death numbers of each group were observed and recorded every 12 hours.At the end of the experiment(96 hours),the half lethal concentrations(LC50)and safe concentration of millettia and liquorice on goldfish and loach were analyzed.The results showed that acute toxicity of liquorice for goldfish was higher than millettia.Conversely,acute toxicity of liquorice for loach was lower than millettia.Further more,the toleration of goldfish to millettia and liquorice was higer than that of loach.The individual half lethal concentrations and safe concentrations of millettia on goldfish and loach were 2.539 0,1.655 0 g.L-1and 0.253 9,0.165 5 g.L-1.Meanwhile,the half lethal concentrations and safe concentrations of liquorice on goldfish and loach were 2.201 0,1.842 0 g.L-1 and 0.220 1,0.184 2 g.L-1 individually.
参考文献/References:
[1]中国兽药典委员会.中华人民共和国兽药典[M].北京:中国农业出版社,2006:193.
[2]陈晓军.鸡血藤的药理作用研究综述[J].中国民族民间医药,2009(13):3-5.
[3]陈道峰,徐国钧,徐珞珊,等.鸡血藤的性状鉴定[J].中药材,1993,16(8):21-23.
[4]崔艳君,陈若芸.鸡血藤化学和药理研究进展[J].天然产物研究与开发,2002,15(4):72-78.
[5]唐勇,何薇,王玉芝,等.鸡血藤黄酮类组分抗肿瘤活性研究[J].中国实验方剂学杂志,2007,13(2):51-54.
[6]王彩兰,韩永生,王艳,等.甘草的调和诸药及解毒作用探讨[J].微量元素与健康研究,1996,13(3):31-32.
[7]梁宁,温海成,陆惠燕,等.壮药鸡血藤提取物毒性作用及体外抗肿瘤作用的实验研究[J].中国民族医药杂志,2010,9(9):3-4.
[8]张瑞梅.甘草的不良反应特点与配伍毒性分析[J].河北中医,2012,34(7):1055-1056.
[9]吴邦灿,费龙.现代环境监测技术[M].北京:中国环境科学出版社,1999:252-254.
[10]刘家国,胡元亮,陈玉库,等.几种天然药物成分在体外CEF中最大安全浓度的测定[J].动物医学进展,2002,23(3):88-91.
[11]惠秀娟.环境毒理学[M].北京:化学工业出版社,2003:266-276.
[12]熊治延.环境生物学[M].武汉:武汉大学出版社,2000:132-137.
[13]崔艳君,刘屏,陈若芸.鸡血藤化学成分研究[J].药学学报,2002,37(10):784.
[14]黄宇玲.从甘草中提取甘草甜素[J].广州食品工业科技,1997,13(3):16-17.
[15]邱招钗.甘草酸的新型提取与精制方法概述[J].福建分析测试,2004,13(3):2058-2062.
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备注/Memo
收稿日期:2014-05-23
基金项目:山西省高等学校大学生创新创业训练资助项目(2013081);山西农业大学科技创新基金资助项目(20132-22);山西水产研究所、山西省水产技术推广站与山西农业大学横向课题资助项目(2013HX86,2013HX102)
第一作者简介:宋艳东(1989-),男,山西省平顺县人,学士,从事动物营养与饲料研究。E-mail:sxausyd@163.com。
通讯作者:刘青(1980-),男,安徽省亳州市人,讲师,博士,从事水产动物健康养殖与育种研究。E-mail:liuqing_sxau@126.com。