[1]董扬,李旭业,杨晓杰,等.不同耐盐碱性玉米自交系过氧化物同工酶分析[J].黑龙江农业科学,2013,(09):5-8.
　DONG Yang,LI Xu-ye,YANG Xiao-jie,et al.The Analysis on Peroxidase Isozyme of Maize Inbred Lines with Different Salt Tolerance[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(09):5-8.
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不同耐盐碱性玉米自交系过氧化物同工酶分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2013年09 页码: 5-8 栏目: 出版日期: 2013-09-30
Title:
The Analysis on Peroxidase Isozyme of Maize Inbred Lines with Different Salt Tolerance
文章编号:
1002-2767（2013）09-0005-04
作者:
董扬1; 李旭业2; 杨晓杰3; 梁虹1; 曲忠诚1; 许健1; 于海林1
1.黑龙江省农业科学院 齐齐哈尔分院，黑龙江 齐齐哈尔 161006；
2.黑龙江省畜牧研究所，黑龙江 齐齐哈尔 161006；
3.齐齐哈尔大学，黑龙江 齐齐哈尔 161006
Author(s):
DONG Yang1; LI Xu-ye2; YANG Xiao-jie3; LIANG Hong1; QU Zhong-cheng1; XU Jian1; YU Hai-lin1
1.Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihar,Heilongjiang 161006;
2.Institute of Animal Husbandry in Heilongjiang Province,Qiqihar,Heilongjiang 161006；
3.Qiqihar University,Qiqihar,Heilongjiang 161006
关键词:
过氧化物同工酶; 盐胁迫; 玉米自交系
Keywords:
peroxidase isozyme; salt tolerance; maize inbred lines
分类号:
S513.034
文献标志码:
A
摘要:
为了有效利用玉米自交系种质资源，促进玉米育种进程，采用凝胶电泳方法研究了32个不同耐盐碱性的玉米自交系过氧化物同工酶的谱带迁移率和聚类分析情况。结果表明：自交系品种过氧化物同工酶谱带位点及带级变化较大，谱带分布不同，且有强弱变化；32个自交系材料共出现258条酶带，大致可划分为3个区：A区（0~0.13），B区（0.26~0.43），C区（0.55~0.81）；过氧化物同工酶的欧氏距离变化范围为0~3.000，平均为1.500；当阈值取20时，供试品种可聚为10类。
Abstract:
In order to use the germplasm resources of maize inbred lines effectively and promote the breeding process,band mobility and cluster analysis of peroxidase isozyme of 32 maize inbred lines with different salt tolerance were studied by gel electrophoresis.The results showed that the band sites and band classes changed greatly,distribution of bands were different and strength change;There were 258 bands which could be divided into three zones:A zone(0～0.13),B zone(0.26～0.43),C zone(0.55～0.81);the change scope of euclidean distance was 0～3.000,the average was 1.500;when the threshold was 20,tested varieties could be clusteredinto 10 types.

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备注/Memo

收稿日期：2013-04-15
第一作者简介：董扬(1982-)，女，黑龙江省齐齐哈尔市人，硕士，研究实习员，从事玉米育种研究。E-mail：hxmlixuye2009@163.com。

更新日期/Last Update: 2014-10-31