[1]周菲.改良CTAB法提取油用向日葵成熟叶片的总RNA[J].黑龙江农业科学,2013,(07):8-9.
　ZHOU Fei.An Improved CTAB Method for Extraction of Total RNA from Mature Leaves in Oil Sunflower[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):8-9.
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改良CTAB法提取油用向日葵成熟叶片的总RNA

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2013年07 页码: 8-9 栏目: 出版日期: 2013-07-30
Title:
An Improved CTAB Method for Extraction of Total RNA from Mature Leaves in Oil Sunflower
文章编号:
1002-2767（2013）07-0008-02
作者:
周菲
黑龙江省农业科学院 经济作物研究所，黑龙江 哈尔滨 150086
Author(s):
ZHOU Fei
Industrial Crops Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086
关键词:
油用向日葵; 成熟叶片; 总RNA提取
Keywords:
oil sunflower; mature leaves; total RNA extraction
分类号:
S565.5
文献标志码:
A
摘要:
为从植物中提取纯度高、完整性好的RNA，采用改良过的CTAB法提取油用向日葵改HA89开花后12 d-〖JP2〗成熟叶片的总RNA，并对其进行了紫外分光光度计和琼脂糖凝胶电泳分析。结果表明：得到的RNA完整性好，纯度和浓度均较高，可用于后续的RT-PCR、Northern杂交和cDNA文库的构建等分子生物学实验研究。
Abstract:
In order to extract RNA of high purity and good integrity from plants,after 12 days’ flowering the total RNA from mature leaves in oil sunflower Modified HA89 was extracted through improved CTAB method.It was analyzed by UV spectrophotometer and agarose gel electrophoresis.The results showed that the structure of total RNA obtained was complete with good purity and high concentration.It could be used for subsequent experiments of molecular biology such as RT-PCR,Northern hybridization,cDNA library construction.

参考文献/References:

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备注/Memo

收稿日期：2013-04-07
基金项目：国家向日葵现代产业技术体系资助项目（CARS-16)
作者简介：周菲（1984-），女，辽宁省丹东市人，硕士，研究实习员，从事向日葵研究。E-mail：zhoufei66666@163.com。

更新日期/Last Update: 2014-10-30