HU Shen,CHENG Yang,LUAN Feng-xia.Research Progress of Detection Methods for Genetically Modified Crops and Products[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(11):135-139.
转基因作物及产品检测技术的研究进展
- Title:
- Research Progress of Detection Methods for Genetically Modified Crops and Products
- 文章编号:
- 1002-2767（2013）11-0135-05
- Keywords:
- genetically modified crops; detection; nucleic acid; DHPLC; LAMP loop-mediated isothermal amplification
- 分类号:
- S188
- 文献标志码:
- A
- 摘要:
- 为了规范管理转基因产品,以建立转基因产品的检测技术标准为前提，通过分析转基因作物商业化的18 a中转基因作物种植面积持续增长的态势，以核酸检测方法为主，介绍了基因芯片、PCR技术、变性高效液相色谱和环介导等温扩增技术在转基因检测领域的研究进展，并对今后转基因检测技术标准的发展进行了展望。
- Abstract:
- In order to standardize the management of genetically modified(GM)products and to create a detection technology,hectares of GM crops has been increased for 18 years since GM crops was commercialized,through the analysis on the growth trend of that,the progress of nucleic acid-based detection methods for GMOs was discussed in the field of GM detection，such as gene chip,PCR,DPLC and LAMP,overall future prospects were also highlighted further.
参考文献/References:
[1]CLIVE J.Global status of commercialized biotech/GM Crops:2012[R/OL].[2013-02-01].http://www.isaaa.org/resources/publications/briefs/44/executivesummary/default.asp.
[2]European Commission Regulation(EC)1829/2003 and1830/2003.Off.J.Eur.Communities[Z].2003,L268:1-28.
[3]中华人民共和国农业部令.农业转基因生物标识管理办法（第10号令）[Z].2002.
[4]Ramsay G.DNA chips:state-of-theart[J].Nature biotechnology,1998,16(1):40-44.
[5]黄文胜，潘良文，粟智平，等.转基因芯片检测转基因油菜[J].农业生物技术学报，2003，11（6）：588-592.
[6]Bai S,Zhang J,Li S,et al.Tection of six genetically modified maize lines using optical thin-film biosensor chips[J].2010,58(15):8490-8494.
[7]刘全振，夏栋，陆利霞，等.转基因食品的检测方法进展[J].食品研究与开发，2007，28（5）：161-164.
[8]周少芸.转基因食品检测方法的研究进展[J].福建稻麦科技，2007，25（1）：43-45.
[9]中华人民共和国国家质量监督检验检疫总局.GB19495-2004，《转基因产品检测》系列标准[S].
[10]农业部.农业部869号公告《转基因植物及其产品成分检测》系列标准[S].
[11]中华人民共和国国家质量监督检验检疫总局.SN/T1202-2010，食品中转基因植物成分定性PCR方法[S]. [12]International Organization for Standardization.ISO 21569:2005,Food stuffs-Methods of analysis for the detection of genetically modified organisms and derived products-Qualitative nucleic acid based methods[S].
[13]Shirai N,Momma K,Ozawa S,et al.Safety Assessment of Genetically Engineered Food:Detection and Monitoring of Glyphosate-Tolerant Soybeans[J].Bioscience Biotechnology and Biochemistry,1998,62(7):1461-1464.
[14]Miehael E R,Tracy S L,Erika M W,et al.Detection and quantification of Round UP Ready soy in foods by conventional and realtime polymerase chain reaction[J].Journal of Agricultural and Food Chemistry,2004,52:5223-5232.
[15]Matsuoka T,Kuribara H,Takubo K,et al.Detection of Recombinant DNA Segments Introduced to Genetically Modified Maize(Zea mays)[J].Journal of Agricultural and Food Chemistry,2002,50:2100-2109. [16]曹际娟,陈明生,卢行安.PCR检测转基因玉米及其粗加工食品[J].玉米科学,2001,9（2）：87-91.
[17]Song P,Cai C Q,Skokut M.Quantitative realtime PCR as a screening tool for estimating transgene copy number in WHISKERSTM derived transgenic maize[J].Plant Cell Reports,2002,20:948-954.
[18]Wurz A,Bluth A P,Zeltz P,et al.Quantitative analysis of genetically modified organisms(GMO)in Processed food by PCR-based methods[J].Food Control,1999,10:385-389.
[19]Christian A H,Junko S.Real Time Quantitative PCR[J].Genome Researeh,1996,6:986-994.
[20]Randhawa G J,Chhabra R,Singh M.Decaplex and real-time PCR based detection of Mon531 and Mon15985 Bt cotton events[J].Journal of Agricultural and Food Chemistry,2010,58(18):9875-9881.
[21]Hird H,Powell J,Johnson M L,et al.Determination of Percentage of Round UP Ready soya in soya flour using greal-time Polymerase chain reaction:inter laboratory study[J].AOAC International，2003,86(1):66-71.
[22]Permingeat H R,Reggiardo M L,Vallejos R H.Detection and quantification of transgenes in grains by multiplex and real-time PCR[J].Journal of agricultural and food chemistry,2002,50(16):4431-4436.
[23]Yuanli Z,Dabing Z,Wenquan L,et al.Anovel real-time quantitative PCR method using attached universal template Probe[J].Nucleic Acids Research,2003,31(20):123.
[24]叶增民，潘婕.转基因大豆及其制品的安全性研究现状[J].生物技术通报,2009(2)：26-28.
[25]Permingeat H R,Reggiardo M L,Vallejos R H.Detection and quantification of transgenes in grains by multiplex and real-time PCR[J].Journal of agricultural and food chemistry,2002,50(16):4431-4436.
[26]白月,栾凤侠.多重PCR结合变性高效液相色谱技术转基因小麦检测方法建立[J].麦类作物学报,2011,31(4)57：577-581.
[27]陶然,郭顺堂.应用MPCR-DHPLC技术检测转基因大豆及其食品[J].大豆科学,2012,31(6):976-979.
[28]王宁,韩金祥.多重PCR归化法平行检测HBV和HCV的研究[J].中华实验和临床病毒学杂志,2003，17（1）：50-54.
[29]况少青，王建民.应用多重PCR进行微卫星荧光标记半自动基因组扫描[J].中华医学遗传学杂志,1998，15（2）：104-107.
[30]Germini A,Zanetti A,Salati C,et al.Development of a seven target multiplex PCR for the simultaneous detecion of transgenie soybean and maize in feed sand foods[J].Journal of Agricultural and Food Chemistry,2004,52(11):3275-3280.
[31]Oefner P J,Underhill P A.Comparative DNA sequencing by denaturing high-performance liquid chromatogra-phy(DHPLC)[J].Amrican Journal of Human Genetics,1995,57(suppl):A266.
[32]Mounier J,Le Blay G,Vasseur V,et al.Application of denaturing high-performance liquid chromatography(DHPLC)for yeasts identification in red smear cheese surfaces[J].Letters in Applied Microbiology,2010,51(1):18-23.
[33]Le Fresne S,Popova M,Le Vacon F,et al.Application of denaturing high-performance liquid chromatography(DHPLC)for the identification of fish:a new way to determine the composition of processed food containing multiple species[J].Journal of Agricultural and Food Chemistry,2011,59(23):12302-12308.
[34]Suwannasri P,Thongnoppakhun W,Pramyothin P,et al.Combination of multiplex PCR and DHPLC-based strategy for CYP2D6 genotyping scheme in Thais[J].Clinical Biochemistry,2011,44(13):1144-1152. [35]Zou H Q,Zhao B J,Yan J,et al.Denaturing high-performance liquid chromatography coupled with multiplex PCR for rapid detection of large duplications or deletions in patients with Duchenne muscular dystrophy and spinal muscular atrophy[J].Zhonghua Yi Xue Yi Chuan Xue Za Zhi.2012,29(6):686-689.
[36]徐君怡,曹际娟,郑秋月.变性高效液相色谱检测沙门氏菌、空肠弯曲菌和肠出血性大肠杆菌[J].生物技术通报,2009(3):127-131.
[37]白月,才华,栾凤侠,等.多重PCR结合DHPLC方法检测番茄中转基因成分[J].作物杂志，2011(2)：28-31.
[38]白月,栾凤侠.多重PCR结合变性高效液相色谱技术转基因小麦检测方法建立[J].麦类作物学报,2011,31(4)：577-581.
[39]白月,栾凤侠,高宏伟.应用多重PCR-DHPLC方法快速检测转基因马铃薯及EH92-527-1品系鉴定[J].中国马铃薯,2011,25(3):129-134.
[40]Feng Xia Luan,Ran Tao,Yi Gang Xu,et al.High-throughput detection of genetically modified rice ingredients in foods using multiplex polymerase chain reaction coupled with high-performance liquid chromatography method[J].European Food Research and Technology,2012,234(4):649-654.
[41]吴静,李铁柱,孙瑶,等.应用PCR-DHPLC技术高通量快速检测转基因玉米[J].玉米科学,2012,20(5):40-44.
[42]Notomi T,Okayama H,Masubuchi H,et al.Loop-mediated isothermal amplification of DNA[J].Nucleic Acids Research,2000,28(12):E63.
[43]Wei H,Zeng J,Deng C,et al.A novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of human astrovirus[J].Journal of virological methods,2013,188(1-2):126-131.
[44]国家质量监督检验检疫总局.SN/T 2754.1~15-2011,出口食品中致病菌环介导恒温扩增（LAMP）检测方法[S].
[45]Vaagt F,Haase I,Fischer M.Loop-mediated isothermal amplification(LAMP)-based method for rapid mushroom species identification[J].Journal of Agricultural and Food Chemistry,2013,61(8):1833-1840.
[46]Focke F,Haase I,Fischer M.Loop-Mediated Isothermal Amplification(LAMP):Methods for Plant Species Identification in Food[J].Journal of Agricultural and Food Chemistry,2013,61(12):2943-2949.
[47]Shiro Fukuta,Yuko Mizukami,Akira Ishida,et al.Real-time loop-mediated isothermal amplification for the CaMV-35S promoter as a screening method for genetically modified organisms[J].European food research and technology,2004,218(5):496-500.
[48]Chen X,Wang X,Jin N.Endpoint visual detection of three genetically modified rice events by loop-mediated isothermal amplification[J].International Journal of Molecular sciences,2012,13(11):14421-14433.
[49]Chen L,Guo J,Wang Q,et al.Development of the visual loop-mediated isothermal amplification assays for seven genetically modified maize events and their application in practical samples analysis[J].Journal of Agricultural and Food Chemistry,2011,59(11):5914-5918.
[50]Liu M,Luo Y,Tao R.Sensitive and rapid detection of genetic modified soybean(Roundup Ready)by loop-mediated isothermal amplification[J].Bioscience,Biotechnology,and Biochemistry,2009,73(11):2365-2369.
[51]叶蕾,沈会平,闫鹤,等.实时浊度LAMP法检测豆制品中转基因成分[J].食品与发酵工业,2012(8)：150-156.
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备注/Memo
收稿日期：2013-05-21
基金项目：国家质检总局科研资助项目(2011IK200)
第一作者简介：胡珅（1982-），女，黑龙江省哈尔滨市人，博士，工程师，从事检验检疫研究。E-mail：hu_cocoa@163.com。
通讯作者：栾凤侠（1963-），女，黑龙江省哈尔滨市人，硕士，教授级高级工程师，从事食品安全和转基因检测方法研究。E-mail：luanfengxia@163.com。