SHI Qun,HE Gui-zheng,CHEN Nai-ming,et al.Study on Industrialization Tissue CultureSeedlings Technology of Cassava[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(11):25-27.
木薯产业化组培育苗技术研究
- Title:
- Study on Industrialization Tissue CultureSeedlings Technology of Cassava
- 文章编号:
- 1002-2767（2012）11-0025-03
- Keywords:
- cassava; tissue culture; micro-cuttage; commercial application
- 分类号:
- S533.043
- 文献标志码:
- A
- 摘要:
- 以“NE199”和“钦州本地”木薯优良品种为材料,进行木薯产业化组培育苗技术研究。结果表明：组培微型扦插培养基使用MS+BA 0.06～0.08 mg·L-1+NAA 0.02 mg·L-1+GA3 0.02 mg·L-1，培养光照强度1 500～3 000 1x，培养温度25~30℃，繁殖系数可达4.0～4.2，腋芽萌芽率达90％以上，玻璃化率和叶片黄化率可控制在2％和6％以内，移栽基质为黄心土∶河沙=8∶1，移栽成活率可达90％以上，可以实现木薯组培育苗产业化生产。
- Abstract:
- Taking ‘NE199’ and ‘the Qinzhou local’ cassava as experiment materials to study industrialization tissue culture seedlings technology.The results showed that:The micro-cuttage medium using MS+BA 0.06~0.08 mg·L-1+NAA 0.02 mg·L-1+GA3 0.02 mg·L-1,light intensity of 1 500~3 000 1x and incubation temperature of 25~30℃,the propagation coefficient could up to 4.0~4.2,axillary bud sprouting rate could reach above 90%,the vitrification rate and leaf yellowing rate could be controlled within 2% and 6%.With the cultivation loess∶sand=8∶1,the transplanting survival rate could reach more than 90%,which could achieve the industrialization of cassava plant tissue culture.
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备注/Memo
基金项目：钦州市科学研究与技术开发计划资助项目（20123602）